Category: Lab Notes

M9 Minimal Salts serve as a base salt solution to prepare M9 Minimal Medium. M9 Minimal salts solution is quite…

Overview: Glucose is a carbohydrate and is utilized by cells as instant energy and carbon source. In addition, it is…

Overview H Medium is a moderately rich medium. It contains tryptone and sodium chloride (NaCl). Tryptone provides basic nutrients and…

Overview: Glucose is a carbohydrate and is utilized by cells as instant energy and carbon source. In addition, it is…

Immunodeficient mice are the most utilized model organisms for the study of several human diseases, especially cancers. The NSG mouse…

If you have a concentrated stock solution of known concentration, you can prepare a working solution of specific concentration and…

Glioma stem cells are a specific type of glioma cell population in glioma tumors that have neural stem cell-like features….

There are several antibodies available that specifically recognize human cells and have no cross-reactivity with well-known animal models like mice…

Overview Tris-EDTA (pH 8.0) solution is used for dissolving and storing DNA. Storing DNA in a slightly alkaline condition reduces…

Overview: Coomassie Brilliant Blue is a protein stain, which is frequently used to visualize protein on acrylamide gels.  Chemically it…

Phosphate buffered saline (PBS) is a buffer solution commonly used in biological research. PBS is isotonic and non-toxic to cells….

Magnetic resonance imaging (MRI)

There are no reports of successful isolation of glioma stem cells from WHO grade I gliomas by neurosphere assay. Several…

Gelatin/Chrome Alum coating helps the slide hold the histological sections firmly, thus reducing the risk of losing histological sections while…

Overview:Chrome alum/gelatin coating helps slide to hold the histological sections firmly, thus reducing the risk of losing histological sections while…