Category: Lab Notes

To cryopreserve, cells from the suspension culture are harvested by centrifugation and resuspended at the correct cell density in an appropriate freezing medium. The resuspension is aliquoted into cryovials and subjected to slow freezing overnight followed by transfer to either the vapor phase of liquid nitrogen or a -150°C freezer. 

Cryopreservation enables long‑term storage of living cells and tissues at ultra‑low temperatures by halting biological time. This article explains freezing damage, cryoprotective agents, slow freezing, vitrification, and natural freeze tolerance.

Cell proliferation refers to a process of cell growth and cell division into two daughter cells. Cell proliferation leads to an exponential increase in cell number and tissue size.