Category: Lab Notes

Mole Fraction

Mole fraction refers to the fraction of a component of a solution when the amounts (component and solution) are expressed…

Preparation of 10X Dulbecco’s Phosphate buffered saline (DPBS)

Overview: Phosphate buffered saline (PBS) is a buffer solution commonly used in biological research. PBS supports cell viability by keeping…

Preparation of 10X Phosphate Buffered Saline (PBS), Sambrook Method

Overview: Phosphate buffered saline (PBS) is a buffer solution commonly used in biological research. PBS supports cell viability by keeping…

Protocol – Plasmid Isolation by Boiling Method (Miniprep)

Overview The boiling lysis method of plasmid isolation is quick and is recommended for the isolation of small plasmids (up…

Preparation of 5M Sodium Chloride (NaCl)

Overview: NaCl is highly soluble in water. Its aqueous solution is clear and colorless. A 5M NaCl solution is easy…

Sodium Deoxycholate

Synonyms Desoxycholic acid sodium salt, 3α,12α-Dihydroxy-5β-cholanic acid sodium salt, 7-Deoxycholic acid sodium salt, Sodium deoxycholate ChemSpider 82577 PubChem CID 23668196…

Vertical Agarose Gel Electrophoresis for Analysis of High Molecular Weight Proteins

SDS-PAGE does not effectively separate proteins larger than 200 kDa. High molecular weight proteins have difficulties moving and entering small…

Neurogenic Niches in Adult Brain

Two main neurogenic niches in the adult brain have been reported:1. Ventricular-subventricular zone (V-SVZ)2. Subgranular zone of the hippocampus (SGZ)In…

Preparation of 1M Calcium chloride (CaCl2) Stock Solution

OVERVIEW Calcium chloride is a salt of calcium and chlorine.  Calcium chloride is available commercially in both anhydrous (CaCl2) and…

Ampicillin Trihydrate

ChemSpider ID22034 PubChem CID 23565 UNII HXQ6A1N7R6 InChIKey RXDALBZNGVATNY-CWLIKTDRSA-N CAS Number 7177-48-2 Molecular formula C16H19N3O4S.3H2O Molecular weight 403.451 g/mol Appearance…

Handling and Aliquoting Frozen Stock of L-glutamine

Ready to use L-glutamine solution (200 mM, sterile-filtered, 100 x concentration) is commercially supplied in different sizes with 100 ml…

Preparation of lysozyme stock solution (10 mg/ml)

Lysozyme is an enzyme used for the extraction of proteins and nucleic acid from bacteria. It improves the extraction process…

Preparation of 0.5 M EDTA Stock Solution from Anhydrous EDTA Free Acid

EDTA (EthyleneDiamineTetraAcetic acid), a polyamino carboxylic acid, is extensively used in molecular biology experiments as a chelating agent. It sequesters…