Category: Lab Notes

Curing Mycoplasma Infected Culture

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Mycoplasma contaminated culture can either be discarded or be cured. The best approach is to discard the culture because: It…

Contaminating Mycoplasma species in Cell Culture

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Several mycoplasma species from human, porcine and bovine origin have been detected in cell culture. The following eight species account…

Preventing Mycoplasma Contamination in Cell Culture

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Mycoplasma contamination is a common and challenging problem in cell culture laboratories, but the following measures can substantially reduce the…

Sources of Mycoplasma Contamination

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Mycoplasma contamination is a prevalent issue in cell culture laboratories, posing significant challenges. However, a comprehensive understanding of mycoplasma contamination…

Common Mycoplasma Species Detected in Cell Culture

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The following species of mycoplasma are most frequently detected in cell culture: Mycoplasma pirum Mycoplasma salivarium Mycoplasma orale Mycoplasma fermentans…

Effects of Mycoplasma on Cultured Cells

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Mycoplasma can affect cell culture in a variety of ways. These are: Morphological changes Slow proliferation rate Reduced saturation density…

Detection of Mycoplasma Contamination

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Detection of mycoplasma is not as easy as detection of bacterial and yeast contamination. Mycoplasma contaminated cultures do not show…

Mycoplasma Contamination in Cell Culture

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Mycoplasma contamination is still a challenging problem in most cell culture laboratories. Due to their small size, they escape microscopic…

Cell Line: HCC827

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HCC827 – A Human epithelial-like lung adenocarcinoma (NSCLC) cell line, established from the lung of a white, 39-year-old female patient with adenocarcinoma.