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Endonuclease Type IIS Restriction enzyme Cleaves double-stranded DNA outside of specific recognition site Recognizes asymmetric DNA sequences (see fig) DNA…

Endonuclease Type IIS Restriction enzyme Cleaves double-stranded DNA outside of specific recognition site Recognizes asymmetric DNA sequences (see fig) DNA…

Sodium chloride (NaCl) is an inorganic compound of two elements: Sodium and Chlorine. The molecular weight of Sodium chloride is 58.44246928 which can be calculated by adding up the total weight (atomic weight multiplied by their number) of Sodium and Chlorine.

Hydrogen fluoride (HF) is an inorganic compound of two elements: Hydrogen, and Fluorine. The molecular weight of Hydrogen fluoride is 20.006403163 which can be calculated by adding up the total weight (atomic weight multiplied by their number) of all its elements.

Isolated from the thermophilic eubacterium Thermus thermophilus HB8 Optimum reaction temperature: 75 °C Optimum pH: ~9.0 (25 °C) Intrinsic reverse…

A 68kDa C-terminal fragment of E. coli DNA Polymerase I Produced by proteolytic cleavage of E. coli DNA polymerase I…

Template-dependent DNA polymerase Catalyzes 5′-3′ synthesis from primed single-stranded DNA Possesses 5’→3′ polymerase activity Possesses 3′-5′ exonuclease activity Lacks 5′-3′…

Isolated from the thermophilic eubacterium Thermus flavus Thermostable DNA polymerase Exhibits a 5′→3′ exonuclease activity Lacks a 3′→5′ exonuclease activity…

Isolated from the thermophilic bacterium Thermus aquaticus Thermostable DNA polymerase Catalyzes 5’→3′ DNA synthesis (5’→3′ polymerase activity) from primed single-stranded…

OVERVIEW Can withstand high temperature Active at high temperature Important tools for polymerase chain reaction Examples of Thermostable DNA Polymerases…

OVERVIEW A 48% (w/w) Hydrofluoric acid can be purchased from several commercial suppliers. It is a clear colorless liquid and…

OVERVIEW A 48% (w/w) Hydrofluoric acid (HF) is a clear colorless aqueous solution of Hydrogen fluoride (HF) gas. It can…

Note: 48% (w/w) Hydrofluoric acid (HF) can be purchased from the following suppliers. You must visit the supplier’s product page…

Endonuclease Type IIS Restriction enzyme Cleaves double-stranded DNA outside of specific recognition site Recognizes asymmetric DNA sequences (see fig) DNA…

OVERVIEW A 70% (w/w) perchloric acid can be purchased from several commercial suppliers. It is a clear colorless liquid and…