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Prolinase
Prolinase (EC 3.4.13.16), also known as proline-specific dipeptidase, is an exopeptidase that catalyzes the hydrolysis of dipeptides with N-terminal proline…
Trypsin
Trypsin is a serine endopeptidase and a key digestive enzyme responsible for cleaving peptide bonds within proteins, specifically at the…
Exopeptidase
Exopeptidases are a class of proteolytic enzymes that catalyze the hydrolysis of peptide bonds at the terminal ends of polypeptide…
Endopeptidase
Endopeptidases, also known as proteases or proteinases, are enzymes that catalyze the hydrolysis of peptide bonds within the interior of…
Matrix Metalloproteinases (MMPs)
Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases that play critical roles in the degradation and remodeling of the…
Antibody Phage Display
Antibody phage display is a powerful in vitro technique used to generate and select antibodies with high affinity and specificity…
Protein Multimerization
Protein multimerization is a fundamental biological process in which individual protein subunits associate to form functional complexes. This phenomenon includes…
Protocol: Analysis of Protein Homodimerization via Differential Tagging and Co-Immunoprecipitation in Mammalian Cells
Overview Homodimerization implies that identical or nearly identical protein monomers associate under physiological conditions. By engineering two expression constructs encoding…
Förster Resonance Energy Transfer (FRET)
Förster Resonance Energy Transfer (FRET) is a highly sensitive and non-invasive technique used to study molecular interactions, particularly protein–protein interactions,…
Bimolecular Fluorescence Complementation (BiFC)
Bimolecular Fluorescence Complementation (BiFC) is a powerful technique used to study protein–protein interactions in living cells by exploiting the reconstitution…
Proximity Labeling
Proximity labeling is a powerful and versatile biochemical approach used to identify and study protein–protein interactions and the molecular composition…
Stromelysin-1
Stromelysin-1, also known as Matrix Metalloproteinase-3 (MMP-3), is an enzyme belonging to the matrix metalloproteinase family. These enzymes play a…
Human Leukocyte Antigen (HLA)/Major Histocompatibility Complex (MHC)
Human Leukocyte Antigen (HLA), also known as the Major Histocompatibility Complex (MHC) in humans, is a complex gene system crucial…
Interferon
Interferons (IFNs) are a family of cytokines that play crucial roles in the immune response, particularly against viral infections. They…
Interleukin
Interleukins (ILs) are a large family of cytokines that play essential roles in the immune system, mediating communication between leukocytes…