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Applications of Cryopreservation
Cryopreservation, the preservation of biological materials at ultra-low temperatures, has become an indispensable tool across a diverse range of fields. …
Liquid Nitrogen in Cryopreservation: An Overview
Liquid nitrogen (LN₂) has become the gold standard for cryopreservation due to its unique properties and advantages. Its extremely low…
Liquid Phase or Vapor Phase – Where to Store Cryopreserved Cells in Liquid Nitrogen?
Cryopreserved cells can be stored in either liquid or vapor phase nitrogen, and choosing between these methods requires careful consideration…
Topic: Cell Culture
Cell Culture Animal Cell Culture Cell Culture: Timeline of Key Events The Origin of Cell Culture: Ross Harrison’s Pioneering…
Poor Trypsinization and Cell Clumps: Impact on Cryopreservation
Poor trypsinization leads to incomplete cell dissociation and clump formation, creating a heterogeneous cell suspension. This initial problem cascades into…
Detrimental Effects of Freezing on Cells and Tissues
The process of freezing can cause extensive damage to cells and tissues through multiple mechanisms. At the cellular level, the…
Gynaephora groenlandica (Arctic Woolly Bear Moth)
Common name: Arctic woolly bear moth Scientific name: Gynaephora groenlandica Classification: Domain: Eukaryota Kingdom: Animalia Phylum: Arthropoda Class: Insecta Order:…
Natural Cryopreservation
Natural cryopreservation is a fascinating biological phenomenon where organisms survive freezing temperatures through evolved adaptations. Some remarkable examples include: Microscopic…
Freezing Aqueous Solution: Understanding the Process
Water freezes at 0°C under normal pressure, forming a hexagonal crystal lattice stabilized by hydrogen bonds. Dissolved substances (solutes) lower…
Water Freezing: Understanding the Process
Pure water freezes at exactly 0°C (32°F) under normal atmospheric pressure. During this process, liquid water transforms into ice, forming…
Impact of Freezing Temperature on Cell Cryopreservation
When temperatures drop below freezing, cells and tissues encounter several challenges, leading to a series of damaging events. As water…
Dimethyl Sulfoxide (DMSO) as Cryoprotectant
Dimethyl Sulfoxide (DMSO) stands as a cornerstone in modern cryobiology, serving as a vital cryoprotectant for preserving biological tissues and…
Glycerol as Cryoprotectant
Glycerol is a commonly used cryoprotectant in the cryopreservation of cells, offering several advantages, especially for cell types sensitive to…
Temperature Effects on Cell Membrane Stability
Cell membrane stability impacts key processes like signal transduction, nutrient uptake, and cell communication. Disruptions in membrane integrity due to…
Effect of Hypothermia on Cells
Cells exposed to hypothermia exhibit several physiological changes, including decreased metabolic rate, reduced enzyme activity, membrane lipid phase transitions, ion…