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Preparation of 6X DNA Loading Dye (Xylene Cyanol FF and Sucrose)
Overview DNA sample is mixed with DNA loading dye prior to loading into the wells of agarose gel. DNA loading…
Preparation of 6X DNA Loading Buffer (Orange G, Xylene Cyanol FF and Ficoll 400)
A 10 ml of 6X DNA loading dye can be prepared by dissolving and mixing 40 mg orange G, 25 mg xylene cyanol FF, 1.5 g Ficoll 400 and 0.1 ml of 1M Tris.Cl, pH 7.6 in sterile deionized or distilled water to a final volume of 10 ml.
Mobility of Xylene Cyanol in Agarose Gel
% agarose gel Mobility of xylene cyanol corresponding to DNA fragment (bp) in 1X TBE buffer Mobility of xylene cyanol…
Suppliers: Power Supply for Electrophoresis
Note: Power Supply for Electrophoresis can be purchased from any of the following suppliers. You must visit the supplier’s product…
Suppliers: Horizontal Electrophoresis System for Agarose Gel Electrophoresis
Note: Horizontal Electrophoresis System can be purchased from any of the following suppliers. You must visit the supplier’s product homepage…
Electrophoresis
Electrophoresis (Electro: electric, Phoresis (greek word): to carry) is the migration of charged molecules or particles under the influence of an electric field. Electrophoresis-based methods are extensively employed for the analysis, separation and purification of biomolecules such as nucleic acid and proteins in laboratories.
Bromophenol Blue
IUPAC name 4,4′-(1,1-dioxido-3H-2,1-benzoxathiole-3,3-diyl)bis(2,6-dibromophenol) Synonyms 3′,3”,5′,5”-tetrabromophenolsulfonephthalein, BPB, Albutest, Tetrabromphenol Blue Chemical formula C19H10Br4O5S Molar mass 669.96 g/mol Molecular weight 669.96 Odor…
Integrin Ligands
List of integrin ligands Bone sialoprotein (BSP) Collagen (extracellular matrix proteins) Cysteine rich protein 61 (CCN1) Developmental locus-1 (Del-1) Elastin…
Water (H20)
Molecular formula H2O CAS Number 7732-18-5 EC Number 231-791-2 PubChem Substance ID 329798917 MDL number MFCD00011332 Molecular (Formula) weight 18.02…
SI base unit
The International System of Units (SI) defines 7 base units as standard units of measurement. They are listed in the…
Water Density
The density of water is dependent on temperature. The maximum density of pure water is 1 g/mL (more precisely 0.999973…
3,3′-Diaminobenzidine (DAB)
3,3′-Diaminobenzidine (DAB) is a chromogenic substrate widely used in immunohistochemistry (IHC), immunocytochemistry (ICC), and blotting techniques for the visualization of…
List of Tight Junction Proteins Between Endothelial Cells of Brain Vasculature
The following tight junction proteins are reported to be present between endothelial cells of brain vasculature: Claudins Claudin-1 Claudin-3 Claudin-5…