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Preparation of 6X DNA Loading Dye (Bromophenol Blue and Glycerol)
Overview DNA loading dye containing only one tracking dye Bromophenol blue (BPB) can be used to analyze larger DNA fragments…
Preparation of 6X DNA Loading Lye (Bromophenol Blue and Ficoll 400)
Overview DNA loading dye containing only one tracking dye bromophenol blue can be used to analyze larger DNA fragments by…
Preparation of 6X DNA Loading Dye (Orange G and Ficoll 400)
Overview DNA loading dye containing only one tracking dye, Orange G, can be used to analyze large and moderate size…
Integrins
Integrins are a large family of type I transmembrane heterodimeric glycoprotein receptors that play central roles in mediating both cell–extracellular…
Mass Spectrometry
Mass spectrometry (MS) is a powerful analytical technique that separates and detects ions based on their mass-to-charge ratio (m/z). This…
Suppliers: Agarose (Standard)
Note: Agarose (standard, CAS No. 9012-36-6) can be purchased from any of the following suppliers. You must visit the supplier’s…
Electroendosmosis in Agarose Gel Electrophoresis
In agarose gel electrophoresis, electroendosmosis (EEO) can be defined as ‘the active water transport towards cathode (negative electrode) from anode…
Cell Line : C6
OVERVIEW Organism Rat (Rattus norvegicus) Source/Tissue N-nitrosomethylurea-induced glioma in brain Morphology Fibroblast-like Cell type Glial cell Growth mode Adherent,…
Preparation of 6X DNA Loading Dye (Xylene Cyanol FF and Ficoll 400)
Overview DNA sample is mixed with DNA loading dye prior to loading into the wells of agarose gel. DNA loading…
Protocol: Size Determination of Plasmid DNA Using Agarose Gel Electrophoresis
Plasmids are circular DNA. Their size can not be determined in their native state as the migration of circular DNA in agarose gel is quite different and can not be compared with the linear fragments of ladder DNA. To know the approximate size of plasmid, the first step is to linearize the plasmid using a restriction enzyme that generates strictly a single cut. This results in a linear DNA that can be run on agarose gel and its size can be compared with the ladder sequence.
Protocol: Size Determination of DNA fragment Using Agarose Gel Electrophoresis
OVERVIEW Approximate size of DNA fragments can easily be determined by agarose gel electrophoresis. This analysis is quite useful to…
Regulation of Anchorage-Independent Growth by Cyclin-dependent Kinase Inhibitors (CKIs)
Cyclin-dependent kinase inhibitors (CKIs), such as p21WAF1, p27KIP1, p16INK4a, or p18INK4c, has been found upregulated in most cell types when…
Regulation of Anchorage-Independent Growth by p27
p27, a member of cyclin-dependent kinase inhibitors (CKIs), is up-regulated in the suspension culture of most cell lines including cancer…
Regulation of Anchorage-Independent Growth by Ras
Expression of dominant active Ras mutant, H-RasV12, has been reported to overcome the requirement of anchorage for a cell to…