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Preparation of Superbroth
Overview Superbroth contains very high-levels of Tryptone and yeast extract that makes it a super rich medium. It can be…
Preparation of TYGPN Medium
OVERVIEW TYGPN is a rich microbial culture medium. It can be prepared by dissolving 20 g Tryptone, 10 g yeast…
Preparation of NZC Broth
OVERVIEW NZC broth is a rich microbial culture medium. It can be prepared by dissolving 10 g NZ Amine A,…
NZ Amine A
NZ Amine is nothing but the end product obtained from enzymatic digestion of bovine milk protein casein. It is enriched…
Preparation of M9 Minimal Medium
OVERVIEW M9 minimal medium is a chemically-defined minimal growth medium, used to cultivate Escherichia coli. It is prepared by supplementing…
Elbing and Brent, 2019. Recipes and Tools for Culture of Escherichia coli. PMID-30412361
Elbing & Brent, 2019. Recipes and Tools for Culture of Escherichia coli. Curr Protoc Mol Biol. 125(1), e83. PMID-30412361; Full-Text…
Preparation of M63 Minimal Medium
OVERVIEW M63 Minimal Medium is a chemically-defined growth medium. It can be used to culture Escherichia coli. It is prepared…
Preparation of 5x A Minimal Medium Without Supplements
OVERVIEW A Minimal Medium is a chemically-defined growth medium. It can be used to culture Escherichia coli. It is prepared…
Preparation of 1 mM Biotin Stock Solution in PBS
OVERVIEW Biotin (also known as vitamin B7) is slightly soluble in water but soluble in hot and alkaline water. A…
Preparation of A Minimal Medium
OVERVIEW A Minimal Medium is a chemically-defined growth medium. It can be used to culture Escherichia coli. It is prepared…
Preparation of 5x M63 Minimal Medium without supplements
OVERVIEW M63 Minimal Medium is a chemically-defined growth medium. It can be used to culture Escherichia coli. It is prepared…
Preparation of 1 mg/ml Thiamine Stock Solution
OVERVIEW Thiamine, also known as vitamin B1, is soluble in water (50 mg/mL) and forms a clear colourless solution. A…
Preparation of 1 mg/ml Biotin Stock Solution in Water
OVERVIEW Biotin (also known as vitamin B7) is slightly soluble in water but soluble in hot and alkaline water. A…
Growth of Escherichia coli in Liquid Medium
To grow Escherichia coli in liquid medium, a small amount of a growth medium (5ml or less) is inoculated with…
Escherichia coli serotype O157:H7
Overview: A member of Enterohaemorrhagic Escherichia coli gram negative, aerobic bacteria Major cause of food associated outbreaks Produces a potent…