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Buffer Name pH range pKa at 25°C ACES 6.1 – 7.5 6.78  ADA 6.7 – 7.2  6.59  AMP 9.0 –…

Template independent DNA polymerase Also known as terminal transferase A member of the X family of DNA polymerase enzymes Add…

Formula: 1 molar (M) = 1000 millimolar (mM) More0.001 molar (M) = 1 millimolar (mM)0.01 molar (M) = 10 millimolar…

Formula: 1 milliliter (mL) = 1000 microliter (µL) More0.001 milliliter (mL) = 1 microliter (µL)0.01 milliliter (mL) = 10 microliter…

Changes in electrophoresis buffer pH is an important parameter to consider when optimizing already existing reagents or conditions or testing…

A 0.5M stock solution of Rubidium chloride (RbCl, Molecular weight = 120.92) can be prepared by dissolving 6.046 g RbCl in deionized water to a final volume of 100 ml.

TA cloning involves base pairing of adenine (A) and thymine (T)  (complementary base pairs) that result in the joining of…

Molecular cloning or DNA cloning refers to a set of methods that involve in generating identical copies of a piece…

High-fidelity thermostable DNA polymerase for error-free polymerase chain reaction. Possess 3′ to 5′ exonuclease activity for proofreading activity Produces blunt-end…

Also known as KOD HiFi DNA Polymerase High-fidelity thermostable DNA polymerase for error-free polymerase chain reaction. Originally isolated from Thermococcus…

High-fidelity thermostable DNA polymerase for error-free polymerase chain reaction. Possess 3′ to 5′ exonuclease activity for proofreading activity Produces blunt-end…

T-vectors are suitable for cloning of DNA fragments that have been amplified by non-proofreading DNA polymerases. These vectors are linear…

IUPAC name 2-[(2-amino-2-oxoethyl)-(carboxymethyl)amino]acetic acid Synonym N-(2-Acetamido)iminodiacetic acid, N-(Carbamoylmethyl)iminodiacetic acid Molecular formula C6H10N2O5 Linear formula H2NCOCH2N(CH2CO2H)2 Molar mass 190.15 g/mol Appearance…

IUPAC name 2-(carbamoylmethylamino)ethanesulfonic acid Synonym N-(2-Acetamido)-2-aminoethanesulfonic acid Molecular formula C4H10N2O4S Molar mass 182.199 g/mol CAS number 7365-82-4 PubChem 81832 ChemSpider…