Category: Lab Notes

A 100 – 200 ng plasmid DNA is sufficient to analyze by restriction digestion. Here we describe a procedure to double digest plasmid DNA with FastDigest EcoRV and HindIII restriction enzymes (from ThermoFisher Scientific). This procedure can be used to check the presence of a DNA fragment cloned in a vector. Double digestion will release the fragment of the appropriate size. 

CAS Number 7681-49-4 Molecular Weight 41.99 InChI 1S/FH.Na/h1H;/q;+1/p-1 InChI key PUZPDOWCWNUUKD-UHFFFAOYSA-M Physical state (Room temperature Solid, powder Colour White

Pyridine (C5H5N) is an organic compound of three elements: Carbon, Hydrogen, and Nitrogen. The molecular weight of Pyridine is 79.10024 which can be calculated by adding up the total weight (atomic weight multiplied by their number) of all its elements.

Sodium molybdate (Na2MoO4) is an inorganic compound of three elements: Sodium, Molybdenum, and Oxygen. The molecular weight of Sodium molybdate is 205.92713856 which can be calculated by adding up the total weight (atomic weight multiplied by their number) of all its elements.

Note: The Sodium Orthovanadate (Na3VO4) can be purchased from the following suppliers. You must visit the supplier’s product page to…

Note: The Protease Inhibitor Cocktail can be purchased from the following suppliers. You must visit the supplier’s product page to…

Sodium sulfate (Na2SO4) is an inorganic compound of three elements: Sodium, Sulfur, and Oxygen. The molecular weight of Sodium sulfate is 142.04313856 which can be calculated by adding up the total weight (atomic weight multiplied by their number) of all its elements.

Gastrin-17 Pepsinogens

Rogosa agar is a selective culture medium for the cultivation of lactobacilli. It can be purchased from several suppliers in…

Nonadecane (C19H40) is an organic compound of two elements: Carbon, and Hydrogen. The molecular weight of Nonadecane is 268.5233 which can be calculated by adding up the total weight (atomic weight multiplied by their number) of all its elements.

The Rogosa agar was originally formulated in 1951 by Rogosa, Mitchell, and Wiseman (Rogosa et al., 1951) to grow lactobacilli…

Tridecane (C13H28) is an organic compound of two elements: Carbon, and Hydrogen. The molecular weight of Tridecane is 184.3631 which can be calculated by adding up the total weight (atomic weight multiplied by their number) of all its elements.

Dodecane (C12H26) is an organic compound of two elements: Carbon, and Hydrogen. The molecular weight of Dodecane is 170.3364 which can be calculated by adding up the total weight (atomic weight multiplied by their number) of all its elements.

Octane (C8H18) is an organic compound of two elements: Carbon, and Hydrogen. The molecular weight of Octane is 114.2296 which can be calculated by adding up the total weight (atomic weight multiplied by their number) of all its elements.

Butane (C4H10) is an organic compound of two elements: Carbon, and Hydrogen. The molecular weight of Butane is 58.1228 which can be calculated by adding up the total weight (atomic weight multiplied by their number) of all its elements.