Category: Lab Notes

Preparation of 10x MOPS Electrophoresis Buffer

Loading

OVERVIEW MOPS is a zwitterionic physiological buffer, with pH range 6.5 – 7.9. MOPS-containing  electrophoresis buffer is suitable for RNA…

Recommended Agarose Gel Percentage for Efficient Separation of DNA Fragments

Loading

Agarose Gel is a porous matrix that acts as a sieve through which negatively charged linear DNA fragments migrate under an…

Cellular Functions of Integrins

Loading

Integrins are reported to regulate the following cellular functions. Cell survival and death Cell cycle progression and proliferation Cell shape…

List of Integrin Subunits

Loading

α1 integrin subunit  α2 integrin subunit  α3 integrin subunit  α4 integrin subunit  α5 integrin subunit  α6 integrin subunit  α7 integrin…

List of Integrin Family Members

Loading

α1β1 integrin  α2β1 integrin  α3β1 integrin  α4β1 integrin  α5β1 integrin  α6β1 integrin  α7β1 integrin  α8β1 integrin  α9β1 integrin  α10β1 integrin …

Integrins in Diseases

Loading

Aberrant integrin signaling is reported in the following diseases Thrombosis  Autoimmune diseases  Cancer

Physiological Functions of Integrins

Loading

Integrins play pivotal roles in the following physiological processes Wound repair Immunity Hemostasis

Preparation of 6X DNA Loading Dye (Xylene Cyanol FF and Sucrose)

Loading

Overview DNA sample is mixed with DNA loading dye prior to loading into the wells of agarose gel.  DNA loading…

Mobility of Xylene Cyanol in Agarose Gel

Loading

% agarose gel Mobility of xylene cyanol corresponding to DNA fragment (bp) in 1X TBE buffer Mobility of xylene cyanol…

Suppliers: Power Supply for Electrophoresis

Loading

Note: Power Supply for Electrophoresis can be purchased from any of the following suppliers. You must visit the supplier’s product…

Suppliers: Horizontal Electrophoresis System for Agarose Gel Electrophoresis

Loading

Note: Horizontal Electrophoresis System can be purchased from any of the following suppliers. You must visit the supplier’s product homepage…

Electrophoresis

Loading

Electrophoresis (Electro: electric, Phoresis (greek word): to carry) is the migration of charged molecules or particles under the influence of an electric field. Electrophoresis-based methods are extensively employed for the analysis, separation and purification of biomolecules such as nucleic acid and proteins in laboratories.

Integrin Ligands

Loading

List of integrin ligands Bone sialoprotein (BSP) Collagen (extracellular matrix proteins) Cysteine rich protein 61 (CCN1) Developmental locus-1 (Del-1) Elastin…

SI base unit

Loading

The International System of Units (SI) defines 7 base units as standard units of measurement. They are listed in the…