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Escherichia coli K-12 (MG1655) Complete Genome

Escherichia coli K-12 (MG1655) complete genome can be found in the link below or click here: https://www.ncbi.nlm.nih.gov/nuccore/U00096

Glioblastoma Stem Cells

It has been shown that Glioblastoma tumors have a subset of tumor cells that resembles closely to neural stem/progenitor cells….

Primary Brain Tumors

Primary brain tumors are tumors that arise within the brain or brain’s immediate surroundings. It can be any tissue located in the brain including central nervous system tissues (gliomas), pituitary gland (pituitary adenomas), pineal gland (pineocytomas), brain blood vessels (hemangioblastomas), etc.

Glioblastoma

Glioblastomas are highly malignant, Grade IV gliomas. They arise in the brain. Depending on their clinical history, they are of…

Anatomic Locations of Gliomas in Brain

The following article describes the anatomical locations of gliomas in the Finnish population. This study included 331 patients.  Larjavaara et…

Preparation of 10X TB salts

Overview: TB salt solution is nothing but a potassium phosphate buffer. A 10x TB salt solution contains 170 mM Potassium…

Preparation of SOC (Super Optimal broth with Catabolite repression) Medium

SOC (Super Optimal broth with Catabolite repression) medium is a nutrient-rich microbial growth medium, formulated by Douglas Hanahan in 1983 (Hanahan, 1983). SOC medium is prepared without Mg++ and Glucose by dissolving 20 g bacto-tryptone, 5 g bacto-yeast extract, 2 ml of 5M sodium chloride, and 2.5 ml of 1M KCl in water to a final volume of ≈ 1000 ml (960 ml). Magnesium salts (i.e., MgSO4 and MgCl2, 10 ml each of 1M MgSO4 and 1M MgCl2) and Glucose (10 ml of 1 M Glucose) are added to the medium after autoclaving to prevent any chemical reactions and precipitation of the medium components, and degradation of Glucose.

Syringe Filters

Syringe filters are disposable devices that are equipped with filter paper. These filter papers are available in different pore sizes,…

Preparation of 1M Magnesium Chloride (MgCl2) Solution from MgCl2.6H2O

Preparation of 1M Magnesium Chloride (MgCl2) Stock Solution

Magnesium Chloride (MgCl2) is soluble in water. Magnesium chloride is available in anhydrous as well as hydrated forms. Since anhydrous Magnesium chloride is hygroscopic (quickly absorbs water from the air), it is convenient to use hydrated forms that are easy to weigh out the right amount accurately. For this reason, Magnesium chloride hexahydrate (MgCl2.​6H2O, Molecular Weight: 203.30) is often used to prepare a 1 M Magnesium chloride solution. A 1M stock solution can be prepared by dissolving 203.3 g of MgCl2.​6H2O (Molecular Weight: 203.30) in water to a final volume of 100 ml. In the following section, we describe a procedure to prepare 100 ml of 1M MgCl2 stock solution.

Preparation of 1M Potassium Chloride (KCl) Stock Solution

Overview: Potassium Chloride (KCl) dissolves readily in water. A 1 M stock solution can be prepared by dissolving 74.55 g…

Preparation of 1M Magnesium Sulfate (MgSO4) Stock Solution

Overview Magnesium sulfate (MgSO4) is soluble in water. Magnesium sulfate is available in anhydrous as well as hydrated forms. Since…

Preparation of Terrific Broth (TB)

Overview: The Terrific Broth was formulated by Tartoff and Hobbs (Tartoff & Hobbs, 1987). It is a rich medium, which…

LB Luria Broth

Preparation of LB Luria Broth

LB is a rich growth medium, commonly used to grow E. coli. LB Luria Broth contains 1% Bacto-tryptone, 0.5% Bacto-yeast extract and 0.05% Sodium chloride. A 1000 ml LB Luria Broth is prepared by dissolving 10 g Bacto-tryptone, 5 g Bacto-yeast extract and 0.5 g sodium chloride in deionized water to a final volume of 1000 ml. After adjusting the pH 7.0 with 5N NaOH medium is autoclaved and stored in a cold room.

Preparation of 5x M9 Minimal Salts

M9 Minimal Salts serve as a base salt solution to prepare M9 Minimal Medium. M9 Minimal salts solution is quite…

Preparation of 20% Glucose Stock Solution

Overview: Glucose is a carbohydrate and is utilized by cells as instant energy and carbon source. In addition, it is…