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Solute
Solute and solvent are the two components of the solution. The dissolved component is called solute whereas the component in…
EcoRV Restriction Enzyme
Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific recognition sites Recognize hexameric palindromic sequence Blunt-end cutter (cut both…
Biological Buffers
Buffer Name pH range pKa at 25°C ACES 6.1 – 7.5 6.78 ADA 6.7 – 7.2 6.59 AMP 9.0 –…
Terminal Deoxynucleotidyl Transferase (TdT)
Template independent DNA polymerase Also known as terminal transferase A member of the X family of DNA polymerase enzymes Add…
Molar – Millimolar Conversion
Formula: 1 molar (M) = 1000 millimolar (mM) More0.001 molar (M) = 1 millimolar (mM)0.01 molar (M) = 10 millimolar…
Calculator: Milliliter – Microliter Conversion
Formula: 1 milliliter (mL) = 1000 microliter (µL) More0.001 milliliter (mL) = 1 microliter (µL)0.01 milliliter (mL) = 10 microliter…
How to Study Changes in Buffer pH During Agarose Gel Electrophoresis?
Changes in electrophoresis buffer pH is an important parameter to consider when optimizing already existing reagents or conditions or testing…
Preparation of 0.5M Rubidium Chloride (RbCl) Stock Solution
A 0.5M stock solution of Rubidium chloride (RbCl, Molecular weight = 120.92) can be prepared by dissolving 6.046 g RbCl in deionized water to a final volume of 100 ml.
TA Cloning
TA cloning involves base pairing of adenine (A) and thymine (T) (complementary base pairs) that result in the joining of…
Molecular Cloning (DNA Cloning)
Molecular cloning or DNA cloning refers to a set of methods that involve in generating identical copies of a piece…
Pfu DNA Polymerase
Pfu DNA polymerase is a high-fidelity, thermostable DNA-dependent DNA polymerase originally isolated from the hyperthermophilic archaeon Pyrococcus furiosus (Lundberg et…
KOD DNA Polymerase
Also known as KOD HiFi DNA Polymerase High-fidelity thermostable DNA polymerase for error-free polymerase chain reaction. Originally isolated from Thermococcus…