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Preparation of 50X TAE Electrophoresis Buffer
A 50x TAE buffer can be prepared by mixing and dissolving 242 g Tris base, 100 ml of 0.5 M EDTA and 57.1 ml glacial acetic acid in a deionized water to a final volume of 1000 ml. The pH of the final solution should be between 8.2 – 8.4.
Preparation of 5X TBE Electrophoresis Buffer
TBE is a very common electrophoresis buffer for DNA agarose gel electrophoresis. It is prepared as a 5X stock solution that can be diluted to 1x or 0.5x working solution for DNA agarose gel electrophoresis. The 5X concentrated solution contains 0.445 M Tris borate and 0.01 M EDTA (pH 8.2 – 8.4).
A 5X stock solution is prepared by dissolving 54 g Tris base, 27.5 g Boric acid, and 20 ml of 0.5 M EDTA in water to a final volume of 1000 ml. The solution is sterilized by autoclaving and is stored at room temperature.
Preparation of Stock Solution of Tetracycline (12 mg/ml)
Overview Tetracycline hydrochloride can be used for biological research. Tetracycline hydrochloride is soluble in water (50 mg/ml) with heating. It…
Ribonuclease A (RNase A) from Bovine Pancreas
Overview CAS Number 9001-99-4 EC Number 232-646-6 Enzyme Commission (EC) Number 3.1.27.5 Synonyms Pancreatic Ribonuclease, RNase A, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase,…
Preparation of Stock Solution of Streptomycin (50 mg/ml)
Overview Streptomycin function by inhibiting protein synthesis in bacteria. Binding of streptomycin to the small 16S rRNA of the 30S…
Preparation of Stock Solution of Kanamycin (50 mg/ml)
Overview Kanamycin is a water-soluble, aminoglycoside bactericidal antibiotic. It was originally purified from the bacterium Streptomyces kanamyceticus. It inhibits protein…
Preparation of LB Lennox Broth
LB medium is a rich growth medium, commonly used to grow E. coli. LB, Lennox Broth contains 1% Bacto-tryptone, 0.5% Bacto-yeast extract and 0.5% Sodium chloride. A 1000 ml LB, Lennox Broth is prepared by dissolving 10 g Bacto-tryptone, 5 g Bacto-yeast extract and 5 g sodium chloride in deionized water to a final volume of 1000 ml. After adjusting the pH 7.0 with 5N NaOH medium is autoclaved and stored in a cold room.
Preparation of Luria-Bertani (LB), Miller Broth
OVERVIEW Luria-Bertani (LB) medium is a rich medium, very commonly used to grow E. coli. It contains tryptone, yeast extract,…
Preparation of Lambda Broth
OVERVIEW Lambda Broth is a moderately rich medium. It is useful in the propagation of λ Phage. It contains tryptone…
Solution
A homogeneous mixture of two or more non-reactive substances is called a solution. A solution is prepared by dissolving one…
Stock Solution
A stock solution can be defined as a highly concentrated solution, mostly prepared as a 10x concentrated solution, which can be diluted to prepare a working solution. Stock solutions can also be used as a component to prepare complex solutions containing many ingredients. For example, a stock solution of EDTA (0.5 M EDTA solution) can be used to prepare many solutions which contain EDTA e.g., TAE, TBE, Tris-EDTA, Trypsin-EDTA etc.
Stability Constants for Metal Ion–EDTA Complexes
Metal Ion-EDTA complex Stability constant (log kf) Al3+–EDTA complex 16.4 Ca2+–EDTA complex 10.65 Cd2+–EDTA complex 16.5 Co2+–EDTA complex 16.45 Co3+–EDTA…
Preparation of Stock Solution of Ethidium Bromide (10 mg/ml)
Overview: Ethidium bromide (EtBr) is a commonly used fluorescent stain to visualize nucleic acid especially DNA in agarose gels. Ethidium…
Molarity of Commercially Supplied Concentrated Acids and Bases
Acids / bases Molecular formula Molecular weight Strength of Concentrated reagent as supplied commercially Specific gravity (g/ml) at 25 °C…
Preparation of Stock Solution of Chloramphenicol (34 mg/ml)
Overview Chloramphenicol is a broad-spectrum antibiotic that is effective against both gram-positive and gram-negative bacteria. It is a bacteriostatic agent….