Author: admin
Preparation of 6X DNA Gel loading buffer (Bromophenol blue, Orange G, Xylene Cyanol FF, EDTA and Ficoll 400)
A 6x DNA Gel loading buffer containing Orange G, Bromophenol blue, Xylene cyanol FF, EDTA and Ficoll 400 is prepared by dissolving 25 mg bromophenol blue, 25 mg xylene cyanol FF, 40 mg Orange G, 1.5 g Ficoll 400 and 1.2 ml of 0.5 M EDTA in 10 mM Tris.Cl, pH 7.6 to a final volume of 10 ml. The final solution (6x DNA loading dye) will contain 0.25% (w/v) bromophenol blue, 0.25% (w/v) xylene cyanol FF, 15% (w/v) Ficoll 400, 60 mM EDTA and 10 mM Tris.