Category: Lab Notes

​​Binding Strength of Protein A/G to Rat Immunoglobulins

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The following Table compares the relative affinity of Protein A and Protein G for Rat immunoglobulins (antibodies).

Binding Strength of Protein A/G to Human Immunoglobulins

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The following Table compares the relative affinity of Protein A and Protein G for human immunoglobulins (antibodies).

Suppliers: Sodium Hydroxide (NaOH) Pellets

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Note: Sodium hydroxide (NaOH) Pellets can be purchased from any of the following suppliers. You must visit the supplier’s product…

Molarity vs Normality of Sodium Hydroxide (NaOH) Solution

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The molarity and normality of the Sodium hydroxide (NaOH) solution are the same which means 1M NaOH solution is also…

Suppliers: 10N (or 10M) Sodium Hydroxide (NaOH) Solution

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Note: The 10N (or 10M) Sodium hydroxide (NaOH) solution can be purchased from the following suppliers. You must visit the…

Topic: Sodium Hydroxide (NaOH)

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NOTES Sodium Hydroxide (NaOH)  Molarity vs Normality of Sodium Hydroxide (NaOH) Solution SOLUTION PREPARATION Preparation of 10 M Sodium Hydroxide…

Co-ImmunoPrecipitation (CO-IP)

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As the name suggests, co-immunoprecipitation employs specific antibodies to sequester a protein of interest together with its interacting partners from…

Preparation of 10x Tris-Glycine Electrotransfer Buffer for Western Blot

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OVERVIEW The 10x Tris-Glycine Transfer Buffer can be used to transfer proteins from polyacrylamide gel to nitrocellulose or PVDF membranes…

Suppliers: 10x SDS-PAGE Running Buffer Containing Tris, Glycine, and SDS

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Note: The 10x SDS-PAGE running buffer can be purchased from the following suppliers. You must visit the supplier’s product page…

tris-base-molecular-weight-calculation

Tris Base [C4H11NO3] Molecular Weight Calculation

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Tris base (C4H11NO3) is an organic compound of four elements: Carbon, Hydrogen, Nitrogen, and Oxygen. The molecular weight of Tris base is 121.13574 which can be calculated by adding up the total weight (atomic weight multiplied by their number) of all its elements.

Preparation of SDS-PAGE Running Buffer (10x)

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SDS-PAGE running buffer contains Tris Base, Glycine, and sodium dodecyl sulfate (SDS). It is used to run protein in SDS-PAGE…

Normality vs Molarity of Hydrochloric Acid

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The normality of Hydrochloric acid is equal to the molarity of Hydrochloric acid which means that a solution of 1M is also a 1N solution of hydrochloric acid.

List of Amino Acids

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Amino acid name  One letter symbol Three letters symbol Chemical formula Molecular weight Alanine  A ala C3H7NO2 89.09 Arginine  R…

Bicine [C6H13NO4] Molecular Weight Calculation

Bicine [C6H13NO4] Molecular Weight Calculation

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Bicine (C6H13NO4) is an organic compound of four elements: Carbon, Hydrogen, Nitrogen, and Oxygen. The molecular weight of Bicine is 163.17254 which can be calculated by adding up the total weight (atomic weight multiplied by their number) of all its elements.

Suppliers: MRS Broth

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Note: The MRS Broth can be purchased from the following suppliers. You must visit the supplier’s product page to order and…