Category: Lab Notes
BseRI Restriction Enzyme
Endonuclease Type IIS Restriction enzyme Cleaves double-stranded DNA outside of specific recognition site Recognizes asymmetric DNA sequences (see fig) DNA…
BsaXI Restriction Enzyme
Endonuclease Type IIS Restriction enzyme Cleaves double-stranded DNA outside of specific recognition site Recognizes asymmetric DNA sequences (see fig) Cleaves…
How to Study Temperature Change During Agarose Gel Electrophoresis?
Temperature change during electrophoresis is an important parameter to consider when optimizing already existing reagents or conditions or testing new…
BaeI Restriction Enzyme
Endonuclease Type IIS Restriction enzyme Cleaves double-stranded DNA outside of specific recognition site Recognizes asymmetric DNA sequences (see fig) Cleaves…
BbsI Restriction Enzyme
Endonuclease Type IIS Restriction enzyme Cleaves double-stranded DNA outside of specific recognition site Recognizes asymmetric DNA sequences (see fig) DNA…
BceAI Restriction Enzyme
Endonuclease Type IIS Restriction enzyme Cleaves double-stranded DNA outside of specific recognition site Recognizes asymmetric DNA sequences (see fig) DNA…
BsmI Restriction Enzyme
Endonuclease Type IIS Restriction enzyme Cleaves double-stranded DNA outside of specific recognition site Recognizes asymmetric DNA sequences (see fig) DNA…
BsmAI Restriction Enzyme
Endonuclease Type IIS Restriction enzyme Cleaves double-stranded DNA outside of specific recognition site Recognizes asymmetric DNA sequences (see fig) DNA…
BsmFI Restriction Enzyme
Endonuclease Type IIS Restriction enzyme Cleaves double-stranded DNA outside of specific recognition site Recognizes asymmetric DNA sequences (see fig) DNA…
Restriction Enzyme
Restriction enzymes belong to a group of enzymes called endonucleases. They are also called restriction endonucleases or restrictase. These enzymes…
AlwI Restriction Enzyme
Endonuclease Type IIS Restriction enzyme Cleaves double-stranded DNA outside of specific recognition site Recognizes asymmetric DNA sequences (see fig) DNA…
Millimolar – Micromolar Conversion
Formula: 1 Millimolar = 1000 Micromolar 0.001 Millimolar = 1 Micromolar More: 0.001 Millimolar = 1 Micromolar 0.01 Millimolar =…
How to Calculate Molarity of 37% (w/w) Hydrochloric acid (HCl), Alternative Method
OVERVIEW Hydrochloric acid is an aqueous solution of hydrogen chloride gas. The density of 37% (w/w) hydrochloric acid solution is…
Percent Agarose Gel
Agarose concentration in a gel is expressed in percentage of agarose to the volume of the buffer (w/v). For example,…
Molar – Micromolar Conversion
Formula: 1 Molar = 1000,000 Micromolar 0.000001 Molar = 1 Micromolar More: 0.000001 Molar = 1 Micromolar 0.00001 Molar =…