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CRISPR Locus

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Please read the following references. REFERENCES Karginov & Hannon, 2010. The CRISPR system: small RNA-guided defense in bacteria and archaea….

Suppliers – Ficoll 400

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Note: Ficoll 400 can be purchased from the following suppliers. You must visit the supplier’s product homepage to order and…

Ficoll 400

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Synonyms Ficoll 400, Polysucrose-400, Ficoll PM 400q CAS Number 26873-85-8 Chemical structure (C12H20O10)n Molecular Weight ≈ 400,000 Appearance White to…

Agarose Gel Casting Set-ups with no requirement of Sealing Edges of Casting Tray with Tape

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Preparing the gel casting tray by sealing both edges (open ends) with tape for agarose gel preparation requires effort. Sometimes agarose solution leaks especially when relatively hot melton agarose is poured into the gel casting tray. To make the agarose gel casting process simple and effortless, many suppliers provide casting tray setups that do not need sealing the edges of casting trays. Some of them are listed in this post.

Suppliers – MOPS Free Acid

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Note: MOPS Free Acid can be purchased from the following suppliers. You must visit the supplier’s product homepage to order…

MOPS (4-Morpholinepropanesulfonic acid) Free Acid

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IUPAC name 3-morpholin-4-yl propane-1-sulfonic acid Synonym 4-Morpholinepropanesulfonic acid3-(N-Morpholino)propanesulfonic acid3-Morpholinopropanesulfonic acidMorpholinopropanesulfonic acid4-Morpholinopropanesulphonic acid4-Morpholinepropane sulfonic acid3-N-Morpholinopropanesulfonic acid Molecular formula C7H15NO4S Molecular weight…

Preparation of 1M MOPS (Free Acid) Solution

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Overview MOPS (Free acid, C7H15NO4S, Molecular weight: 209.26) is a zwitterionic buffer, useful for several cell and molecular biology applications…

Preparation of 10x MOPS Electrophoresis Buffer

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OVERVIEW MOPS is a zwitterionic physiological buffer, with pH range 6.5 – 7.9. MOPS-containing  electrophoresis buffer is suitable for RNA…

Recommended Agarose Gel Percentage for Efficient Separation of DNA Fragments

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Agarose Gel is a porous matrix that acts as a sieve through which negatively charged linear DNA fragments migrate under an…

Molecular Weight and Equivalent Weight of Common Acids and Bases

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Chemical name Formula Number of dissociable H+/OH— (valence) in solution Molar mass (= weight weight) g/mol Equivalent weight (g/eq) Hydrochloric…

Cellular Functions of Integrins

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Integrins are reported to regulate the following cellular functions. Cell survival and death Cell cycle progression and proliferation Cell shape…

List of Integrin Subunits

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α1 integrin subunit  α2 integrin subunit  α3 integrin subunit  α4 integrin subunit  α5 integrin subunit  α6 integrin subunit  α7 integrin…

List of Integrin Family Members

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α1β1 integrin  α2β1 integrin  α3β1 integrin  α4β1 integrin  α5β1 integrin  α6β1 integrin  α7β1 integrin  α8β1 integrin  α9β1 integrin  α10β1 integrin …

Integrins in Diseases

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Aberrant integrin signaling is reported in the following diseases Thrombosis  Autoimmune diseases  Cancer

Physiological Functions of Integrins

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Integrins play pivotal roles in the following physiological processes Wound repair Immunity Hemostasis