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β-Lactam Antibiotics
β-Lactam antibiotics are a major class of antibiotics defined by their distinctive β-lactam ring structure. These medications have revolutionized the…
Ampicillin Sodium Salt
Synonym D-(−)-α-Aminobenzylpenicillin sodium saltSodium ampicillinAmpicillin sodiumAmpicillin sodium salt CAS Number 69-52-3 Beilstein Registry Number 4119211 EC Number 200-708-1 MDL number…
Ampicillin
Ampicillin belongs to a group of beta-lactam antibiotics. It is classified as critically important human medicine by WHO. Ampicillin is one of the synthetic derivatives of penicillin. It was generated by adding an amino group side chain on penicillin. Ampicillin inhibits the synthesis of the bacterial cell wall by stopping the peptidoglycan cross-linking. It is effective against both gram-positive and gram-negative bacteria.
Recommended Antibiotic Concentrations for the Selection and Growth of Escherichia coli Carrying a Plasmid
Most of the commonly used plasmid vectors carry at least one antibiotic resistance gene that allows plasmid-containing bacterial cells to…
PROTACs (PROteolysis TArgeting Chimeras)
Read the following reviews to know about PROTACs. ♦ Pettersson & Crews, 2019. PROteolysis TArgeting Chimeras (PROTACs) – Past, present…
Protocol – RNA Isolation from Tissue Using Trizol
Overview: ♦ Tissues are composed of multiple cell layers that are tightly glued together by extracellular matrix. ♦ Solubilizing all…
Protocol – RNA Isolation from Suspension Cell Culture Using Trizol
Overview: Cell lines are a very useful tool for life science research. Isolation of total RNA from cell lines is…
Protocol – RNA Isolation from Escherichia coli Using Trizol
Overview: Escherichia coli (E. coli) is one of the well characterized bacteria and is extensively used in both applied and…
Protocol – RNA Isolation from Adherent Monolayer Cell Culture Using Trizol
Overview: Cell lines are very useful tools for life science research. Isolation of total RNA from cell lines is often…
Commonly Used Cell Culture Vessels
Culture vessel types Surface area Culture flask T-25 25 cm2 T-75 75 cm2 T-175 175 cm2 Culture dish 35 mm…
List of Monophasic Solution for RNA Extraction
TRIzol ™ Reagent (Invitrogen) Supplied by ThermoFisher Scientific TRI Reagent® Supplied by MRC, Sigma Aldrich, Zymoresearch RNAzol® (Tel-Test) RNA Stat-60…
Isolation of RNA Using Trizol
Trizol is a very popular reagent among researchers for RNA isolation due to the simplicity and reliability of the isolation…
Protocol – Preparing RNA free of DNA Using DNase I Digestion
Overview DNase I is an endonuclease that selectively cleaves phosphodiester bonds of both single- and double-stranded DNA without cleaving RNA….