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Nestin
Nestin is an intermediate filament protein Encoded by the NES gene (in human) Found in undifferentiated central nervous system cells…
Preparation of LB-Agar Medium and Plates
OVERVIEW LB medium can be solidified by adding agar. Solidified culture medium is very useful for the selection and identification…
Preparation of 6X DNA Loading Dye (Bromophenol blue and Sucrose)
Overview DNA sample is mixed with the DNA loading dye prior to loading into the wells of agarose gel. A…
Collagen Binding Integrins
The following integrins act as receptors of collagen. α1β1 (Major receptor) α2β1 α10β1 α11β1
Extracellular Matrix (ECM)
The extracellular matrix (ECM) is a dynamic and complex network of macromolecules that provides structural and biochemical support to surrounding…
Preparation of 6X DNA Loading Dye (Bromophenol blue, xylene cyanol FF and Sucrose) Alternative Method
OVERVIEW This method uses 40% sucrose solution instead of sucrose powder. This method is convenient and easy, and does not…
Preparation of 6X DNA Loading Dye (Bromophenol blue and Sucrose) Alternative Method
OVERVIEW This method uses 40% sucrose solution instead of sucrose powder. This method is convenient and easy, and does not…
Preparation of 6x DNA Loading Dye (Xylene Cyanol FF and Sucrose) Alternative Method
OVERVIEW This method uses 40% sucrose solution instead of sucrose powder. This method is convenient and easy, and does not…
Preparation of 6X DNA Loading Dye (Orange G and Sucrose)
Overview DNA loading dye containing only one tracking dye, Orange G, can be used to analyze large and moderate size…
Preparation of 6X DNA Loading Dye (Orange G and Sucrose) Alternative Method
OVERVIEW This method uses 40% sucrose solution instead of sucrose powder. This method is convenient and easy, and does not…
High-density Reagents for DNA Loading Dye
DNA loading dye contains a high density reagent. The high-density reagent is added to facilitate the sample to be placed…
Tracking dyes for Agarose Gel Electrophoresis
There are several dyes (Bromophenol blue, Bromocresol green, Orange G, Xylene cyanol FF) that can be used for the preparation…
World Health Organization (WHO) Classification of Central Nervous System Tumors
WHO has classified the central nervous tumors into distinct groups. The first classification was published in 1979. The classification has…
Protocol: Visualizing DNA Bands in Agarose Gel Using GelRed – Precast Method
GelRed is a DNA staining dye and can be used to visualize DNA embedded in agarose gel. Both precast (GelRed…
Preparation of 6X DNA Loading Lye (Xylene Cyanol FF and Glycerol)
Overview DNA loading dye containing only one tracking dye, xylene cyanol FF, can be used to analyze smaller DNA fragments…