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GBM14
Human patient-derived glioma xenolines (Watkins et al., 2014) REFERENCES: Watkins et al., 2014. Disruption of astrocyte-vascular coupling and the blood-brain…
GBM39
Human patient-derived glioma xenolines (Watkins et al., 2014) REFERENCES: Watkins et al., 2014. Disruption of astrocyte-vascular coupling and the blood-brain…
Blood-Brain Barrier (BBB)
The BBB is a highly specialized structure in the Central nervous system (CNS) between the blood circulation and the brain…
Protocol – Growing Large Volume of Liquid Culture of Escherichia coli for Large-Scale Plasmid Isolation
Large-scale isolation of plasmid requires a large volume of E. coli culture. Large scale plasmid isolation procedures are termed, midiprep (25 – 50 ml starting culture volume) and maxiprep (100 – 500 ml starting culture volume). A starter culture is initially prepared by inoculating a colony in a small volume (2 – 10 ml) of culture medium. Large culture volume is prepared by diluting starter culture in a ratio of 1: 100 to 1: 1000 in the growth medium.
GBM22
Human patient-derived glioma xenolines (Watkins et al., 2014) REFERENCES: Watkins et al., 2014. Disruption of astrocyte-vascular coupling and the blood-brain…
Cell Line : D54MG
Human glioma cell line (Watkins et al., 2014) REFERENCES: Watkins et al., 2014. Disruption of astrocyte-vascular coupling and the blood-brain…
D54MG Glioma Cell Line-Derived Brain Tumors in Immunodeficient Mice
Transplantation of 1.25 – 5.0 x 105 cells in the brain of adult 6 – 12 weeks old immunodeficient C.B.17…
Interaction of Glioma Cells with Pre-existing Blood Vessels (Vessel Co-option)
The interaction of glioma cells with blood vessels has a high impact on glioma development, therefore has been considered as…
Glioma Cell Lines that Show Extensive Perivascular Invasion in Rodent Brain
The following cell lines have been shown to invade perivascularly when transplanted into the rodent brain. D54 (Watkins et al.,…
Preparation of Lambda-agar medium and plates
Lambda-agar is nothing but a lambda broth which is solidified by agar. Agar is a solidifying agent. It melts at 85°C but melton agar stays liquid at a temperature > 40°C (this property is called hysteresis, different temperatures for melting and solidifying). This allows us to add heat-labile medium supplements such as antibiotics and vitamins and prepare solid medium. Lambda-agar solid medium is prepared by dissolving 15 g agar / liter of Lambda broth.
Preparation of TNT Medium
OVERVIEW TNT medium is a moderately rich medium. It contains tryptone, sodium chloride (NaCl) and thiamine. Tryptone provides basic nutrients…
Methods for Detecting Antibiotic-resistant Bacteria
Please read the following peer-reviewed articles to know different methods to detection of antibiotic-resistant bacteria: Ash et al., 2002. Antibiotic…