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Protocol: Preparing T-vector using Terminal Transferase and ddTTP
T-vectors are linearized vectors that contain a single T-overhang at the 3’-end of both DNA strands. T-vectors are useful for…
XbaI Restriction Enzyme
Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific sites Recognize hexameric palindromic sequence Sticky end cutter (cut both…
BamHI Restriction Enzyme
Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific recognition site Recognize hexameric palindromic sequence Sticky end cutter (cut…
KpnI Restriction Enzyme
Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific recognition site Recognize hexameric palindromic sequence Sticky end cutter (cut…
XhoI Restriction Enzyme
Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific recognition site Recognize hexameric palindromic sequence Sticky end cutter (cut…
Suppliers: 45% (w/w) Potassium Hydroxide Solution in Water
Note: A 45% (w/w) Potassium hydroxide solution can be purchased from the following suppliers. You must visit the supplier’s product…
Suppliers: 95% (w/w) Sulfuric Acid Solution
Note: A 95% (w/w) Sulfuric acid solution can be purchased from the following suppliers. You must visit the supplier’s product…
Solute
Solute and solvent are the two components of the solution. The dissolved component is called solute whereas the component in…
EcoRV Restriction Enzyme
Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific recognition sites Recognize hexameric palindromic sequence Blunt-end cutter (cut both…
Biological Buffers
Buffer Name pH range pKa at 25°C ACES 6.1 – 7.5 6.78 ADA 6.7 – 7.2 6.59 AMP 9.0 –…
Terminal Deoxynucleotidyl Transferase (TdT)
Template independent DNA polymerase Also known as terminal transferase A member of the X family of DNA polymerase enzymes Add…