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Preparation of Lambda-agar medium and plates
Lambda-agar is nothing but a lambda broth which is solidified by agar. Agar is a solidifying agent. It melts at 85°C but melton agar stays liquid at a temperature > 40°C (this property is called hysteresis, different temperatures for melting and solidifying). This allows us to add heat-labile medium supplements such as antibiotics and vitamins and prepare solid medium. Lambda-agar solid medium is prepared by dissolving 15 g agar / liter of Lambda broth.
Preparation of TNT Medium
OVERVIEW TNT medium is a moderately rich medium. It contains tryptone, sodium chloride (NaCl) and thiamine. Tryptone provides basic nutrients…
Methods for Detecting Antibiotic-resistant Bacteria
Please read the following peer-reviewed articles to know different methods to detection of antibiotic-resistant bacteria: Ash et al., 2002. Antibiotic…
Preparation of Superbroth
Overview Superbroth contains very high-levels of Tryptone and yeast extract that makes it a super rich medium. It can be…
Preparation of TYGPN Medium
OVERVIEW TYGPN is a rich microbial culture medium. It can be prepared by dissolving 20 g Tryptone, 10 g yeast…
Preparation of NZC Broth
OVERVIEW NZC broth is a rich microbial culture medium. It can be prepared by dissolving 10 g NZ Amine A,…
NZ Amine A
NZ Amine is nothing but the end product obtained from enzymatic digestion of bovine milk protein casein. It is enriched…
Preparation of M9 Minimal Medium
OVERVIEW M9 minimal medium is a chemically-defined minimal growth medium, used to cultivate Escherichia coli. It is prepared by supplementing…
Elbing and Brent, 2019. Recipes and Tools for Culture of Escherichia coli. PMID-30412361
Elbing & Brent, 2019. Recipes and Tools for Culture of Escherichia coli. Curr Protoc Mol Biol. 125(1), e83. PMID-30412361; Full-Text…
Preparation of M63 Minimal Medium
OVERVIEW M63 Minimal Medium is a chemically-defined growth medium. It can be used to culture Escherichia coli. It is prepared…
Preparation of 5x A Minimal Medium Without Supplements
OVERVIEW A Minimal Medium is a chemically-defined growth medium. It can be used to culture Escherichia coli. It is prepared…
Preparation of 1 mM Biotin Stock Solution in PBS
OVERVIEW Biotin (also known as vitamin B7) is slightly soluble in water but soluble in hot and alkaline water. A…
Preparation of A Minimal Medium
OVERVIEW A Minimal Medium is a chemically-defined growth medium. It can be used to culture Escherichia coli. It is prepared…
Preparation of 5x M63 Minimal Medium without supplements
OVERVIEW M63 Minimal Medium is a chemically-defined growth medium. It can be used to culture Escherichia coli. It is prepared…
Preparation of 1 mg/ml Thiamine Stock Solution
OVERVIEW Thiamine, also known as vitamin B1, is soluble in water (50 mg/mL) and forms a clear colourless solution. A…