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List of Monophasic Solution for RNA Extraction
TRIzol ™ Reagent (Invitrogen) Supplied by ThermoFisher Scientific TRI Reagent® Supplied by MRC, Sigma Aldrich, Zymoresearch RNAzol® (Tel-Test) RNA Stat-60…
Isolation of RNA Using Trizol
Trizol is a very popular reagent among researchers for RNA isolation due to the simplicity and reliability of the isolation…
Protocol – Preparing RNA free of DNA Using DNase I Digestion
Overview DNase I is an endonuclease that selectively cleaves phosphodiester bonds of both single- and double-stranded DNA without cleaving RNA….
Preparing RNA free of DNA
DNA-free RNA preparations are absolutely required for applications like gene expression analysis using PCR-based methods (e.g., real-time PCR). No RNA…
Preparation of 1M IPTG Solution
Overview: In molecular biology laboratories, IPTG is commonly used to induce the expression of recombinant proteins in E. coli and…
Cell Line : B-16V
Overview Organism Mouse (Mus musculus) Source/Tissue Melanoma Morphology Epithelial-like Cell type Growth mode Adherent, monolayer Doubling time ≈…
Cell Line : A-204
OVERVIEW Organism Human (Homo sapiens), 1 year old, female Source/Tissue Muscle, rhabdomyosarcom Morphology Epithelial-like Cell type Growth mode…
Preparation of Tris-saturated Phenol
Overview: Phenol is a colorless crystalline solid. Phenol gradually turns pink to brownish color due to oxidation upon exposure to…
Preparation of 4% Paraformaldehyde Solution in PBS
A 100 ml of 4% (w/v) paraformaldehyde can be prepared by dissolving 4g of paraformaldehyde in PBS to a final volume of 100 ml.
Detection of DNA in Agarose Gel
♦ After the gel electrophoresis has been completed, the next step is to analyze agarose gel for DNA fragments. Unfortunately,…
Nucleic Acid Staining Dyes for Detection of DNA in Agarose Gel
There are many nucleic acid staining dyes that can be used to visualize DNA embedded in the agarose gel. These…
Preparation of Metaphase Chromosome Spreads from Adherent Cell Line
Overview Preparation of metaphase chromosome spread is an important technique in cytogenetics. Metaphase chromosome spread is used for karyotyping including…
Handling and Aliquoting Frozen Stock of Serum
The serum is commercially supplied in different sizes with 500 ml being the most common size. Since repeated freezing and…
Handling and Aliquoting Frozen Stock of L-glutamine
Ready to use L-glutamine solution (200 mM, sterile-filtered, 100 x concentration) is commercially supplied in different sizes with 100 ml…