Category: Lab Notes
ExpSup: Membrane Localization of CagA is Independent of Tyrosine Phosphorylation
The following experimental approaches support the notion that “Membrane localization of CagA is independent of tyrosine phosphorylation”. Immunofluorescence analysis of…
Ref: SHP-2 Tyrosine Phosphatase as an Intracellular Target of Helicobacter pylori CagA Protein.
Higashi et al., 2002. SHP-2 tyrosine phosphatase as an intracellular target of Helicobacter pylori CagA protein. Science. 295(5555), 683-686. PMID-11743164;…
Epidemiology
Epidemiology is the study of how diseases and other health-related conditions are distributed in populations, and the factors that influence…
Glossary
Glossary Glossary: 0-9 λDE3 Lysogen Glossary: A araBAD Promoter lossary: B Glossary: C Chemotaxis Casein Zymography Caseinolytic activity Caseinolytic Proteases …
Fibrin Zymography
Fibrin zymography is a gel-based electrophoretic technique used to detect and analyze fibrinolytic enzymes—those that can degrade fibrin, the main…
Fibrinolytic Activity
Fibrinolytic activity refers to the body’s natural ability to break down fibrin, the protein mesh that stabilizes blood clots. This…
Casein Zymography
Casein zymography is a technique used to detect casein-degrading enzymes in biological samples. Procedure Preparation: A polyacrylamide gel is co-polymerized…
Caseinolytic activity
Caseinolytic activity refers to the ability of proteases to degrade casein, a milk-derived phosphoprotein. This property is commonly used to…
Zymography
Zymography is a sensitive, semi-quantitative electrophoretic technique used to detect and analyze the enzymatic activity of proteases, especially matrix metalloproteinases…
Tight Junctions in Epithelial-to-Mesenchymal Transition (EMT)
Tight junctions are specialized cell-cell adhesion structures that play a fundamental role in maintaining the polarity and integrity of epithelial…
Topic: Epithelial-to-Mesenchymal Transition (EMT)
Epithelial-to-Mesenchymal Transition (EMT) Key Indicators for Monitoring Epithelial-to-Mesenchymal Transition (EMT) Tools for Studying Epithelial-to-Mesenchymal Transition (EMT) Matrix Metalloproteinase-3 (MMP-3) in…
Helicobacter pylori CagA protein induces factors involved in the epithelial to mesenchymal transition (EMT) in infected gastric epithelial cells in an EPIYA- phosphorylation-dependent manner.
Sougleri et al., 2016. Helicobacter pylori CagA protein induces factors involved in the epithelial to mesenchymal transition (EMT) in infected…
Effect of CagA on β-Catenin Signaling in AGS Cells
Although AGS cells do not express functional E-cadherin, Helicobacter pylori can still modulate β-catenin signaling in these cells through E-cadherin–independent…
E-cadherin Expression in AGS Cell Line
AGS cells, a widely used human gastric adenocarcinoma cell line, do not express functional E-cadherin due to epigenetic silencing of…
β-Catenin Expression in AGS Cell Line
In normal epithelial cells, β-catenin is sequestered at the adherens junctions via interaction with E-cadherin, helping maintain junctional integrity and…