Category: Lab Notes
Topic: Cell Culture
Cell Culture Animal Cell Culture Cell Culture: Timeline of Key Events The Origin of Cell Culture: Ross Harrison’s Pioneering…
Poor Trypsinization and Cell Clumps: Impact on Cryopreservation
Poor trypsinization leads to incomplete cell dissociation and clump formation, creating a heterogeneous cell suspension. This initial problem cascades into…
Detrimental Effects of Freezing on Cells and Tissues
The process of freezing can cause extensive damage to cells and tissues through multiple mechanisms. At the cellular level, the…
Gynaephora groenlandica (Arctic Woolly Bear Moth)
Common name: Arctic woolly bear moth Scientific name: Gynaephora groenlandica Classification: Domain: Eukaryota Kingdom: Animalia Phylum: Arthropoda Class: Insecta Order:…
Natural Cryopreservation
Natural cryopreservation is a fascinating biological phenomenon where organisms survive freezing temperatures through evolved adaptations. Some remarkable examples include: Microscopic…
Freezing Aqueous Solution: Understanding the Process
Water freezes at 0°C under normal pressure, forming a hexagonal crystal lattice stabilized by hydrogen bonds. Dissolved substances (solutes) lower…
Water Freezing: Understanding the Process
Pure water freezes at exactly 0°C (32°F) under normal atmospheric pressure. During this process, liquid water transforms into ice, forming…
Impact of Freezing Temperature on Cell Cryopreservation
When temperatures drop below freezing, cells and tissues encounter several challenges, leading to a series of damaging events. As water…
Dimethyl Sulfoxide (DMSO) as Cryoprotectant
Dimethyl Sulfoxide (DMSO) stands as a cornerstone in modern cryobiology, serving as a vital cryoprotectant for preserving biological tissues and…
Glycerol as Cryoprotectant
Glycerol is a commonly used cryoprotectant in the cryopreservation of cells, offering several advantages, especially for cell types sensitive to…
Temperature Effects on Cell Membrane Stability
Cell membrane stability impacts key processes like signal transduction, nutrient uptake, and cell communication. Disruptions in membrane integrity due to…
Effect of Hypothermia on Cells
Cells exposed to hypothermia exhibit several physiological changes, including decreased metabolic rate, reduced enzyme activity, membrane lipid phase transitions, ion…
Impact of Hypothermia on Cell Preservation
When cells are exposed to hypothermia (chilling to non-freezing temperatures), they undergo several structural and physiological changes. Prolonged exposure to…
Dynamic Nature of Cells in Culture
Cell culture is a dynamic and evolving process, where cells are not static entities but rather active participants in their…
Phage Display
Phage display is a powerful laboratory technique used to study protein interactions. Phage display involves expressing proteins or peptides of…