Category: Lab Notes
AGS Cell Line: Gene Expression Profile
It lacks E-cadherin expression It expresses ɑ-catenin, β-catenin, and p120-catenin.
ExpSup: AGS Cell Line Does Not Express E-cadherin
Hsieh et al., 1996 analyzed the surface expression of E-cadherin by flow cytometry in untreated cells and treated with TNFɑ,…
ExpSup: Hummingbird Phenotype is Induced only by CagA-positive Helicobacter pylori, but not by CagA-negative Helicobacter pylori
AGS cells acquire hummingbird phenotype when infected with CagA-positive H. pylori strain G27 but not with CagA-negative H. pylori strain…
AGS Cell Line: Subculturing Procedure
OVERVIEW AGS cells grow as an adherent monolayer. Therefore, detaching cells from the substrate using specific means is required for…
AGS Cell Line: Culture Media
Various culture media have been used to maintain the AGS cell line in culture and for specific experimental procedures. A 10% fetal bovine serum (FBS)-supplemented RPMI medium is the most popular medium used by many researchers to maintain AGS cells in culture. Some have also used 10% FBS-supplemented DMEM. ATCC suggests Ham’s F12K Medium supplemented with 10% FBS. A list of base media used for AGS cell line, experiment-specific supplements, culture conditions, purpose, and references are given in the table below.
Aspartic acid [C4H7NO4] Molecular Weight Calculation
The molecular weight of Aspartic acid [C4H7NO4] is 133.10314. Aspartic acid (C4H7NO4) is an organic compound of four elements: Carbon, Hydrogen,…
Adenosine Triphosphate (ATP) [C10H16N5O13P3] Molecular Weight Calculation
The molecular weight of Adenosine triphosphate (ADP) [C10H15N5O10P2] is 507.182185994. Adenosine triphosphate (C10H15N5O10P2) is an organic compound of five elements:…
Adenosine Diphosphate (ADP) [C10H15N5O10P2] Molecular Weight Calculation
The molecular weight of Adenosine diphosphate (ADP) [C10H15N5O10P2] is 427.202223996. Adenosine diphosphate (C10H15N5O10P2) is an organic compound of five elements:…
Suppliers: Triton X-100
Note: Triton X-100 can be purchased from the following suppliers. You must visit the supplier’s product page to order and…
Triton X-100
Synonyms X-100, TRITON X-100, Octylphenol ethylene oxide condensate CAS 9002-93-1 Appearance Liquid, clear to slightly hazy, colorless to light yellow…
Suppliers: Tween 20
Note: Tween 20 can be purchased from the following suppliers. You must visit the supplier’s product page to order and…
Tween 20
Synonyms Polysorbate 20; PEG(20)sorbitan monolaurate, polyoxyethylenesorbitan monolaurate CAS Number 9005-64-5 Boiling point >100 °C Brookfield Viscosity 370-430 cps (25 °C,…
Alexa Fluor 500
Absorption maxima (wavelength) (nm) 503 Emission maxima (wavelength) (nm) 525 Color (as visible to the human eye) Green Extinction coefficient…
Alexa Fluor 488
Absorption maxima (wavelength) (nm) 496 Emission maxima (wavelength) (nm) 519 Color (as visible to the human eye) Green Extinction coefficient…
Alexa Fluor 430
Absorption maxima (wavelength) (nm) 433 Emission maxima (wavelength) (nm) 541 Color (as visible to the human eye) Green/Yellow Extinction coefficient…