Category: Lab Notes
Millimolar – Nanomolar Conversion
Formula: 1 Millimolar = 1000,000 Nanomolar 0.000001 Millimolar = 1 Nanomolar More 0.000001 Millimolar = 1 Nanomolar 0.00001 Millimolar =…
Nitrogenous Bases
Nitrogenous bases (also known as Nucleobases or bases) are nitrogen-containing compounds. They are an important component of nucleic acids. These…
EcoRI Restriction Enzyme
Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific cleavage sites Recognize hexameric palindromic sequence Sticky end cutter (cuts…
PmeI Restriction Enzyme
Synonyms: MssI Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific sites Recognize octameric palindromic sequence Blunt-end cutter (cut…
NheI Restriction Enzyme
Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific sites Recognize hexameric palindromic sequence Sticky end cutter (cut both…
SmaI Restriction Enzyme
Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific recognition site Recognize hexameric palindromic sequence Blunt-end cutter (cut both…
NotI Restriction Enzyme
Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific recognition site Recognize octameric palindromic sequence Sticky end cutter (cut…
Protocol: Preparing T-vector using Terminal Transferase and ddTTP
T-vectors are linearized vectors that contain a single T-overhang at the 3’-end of both DNA strands. T-vectors are useful for…
XbaI Restriction Enzyme
Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific sites Recognize hexameric palindromic sequence Sticky end cutter (cut both…
BamHI Restriction Enzyme
Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific recognition site Recognize hexameric palindromic sequence Sticky end cutter (cut…
KpnI Restriction Enzyme
Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific recognition site Recognize hexameric palindromic sequence Sticky end cutter (cut…
XhoI Restriction Enzyme
Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific recognition site Recognize hexameric palindromic sequence Sticky end cutter (cut…
Suppliers: 45% (w/w) Potassium Hydroxide Solution in Water
Note: A 45% (w/w) Potassium hydroxide solution can be purchased from the following suppliers. You must visit the supplier’s product…