Category: Lab Notes
Protocol: Visualizing DNA in agarose gel using ethidium bromide – Precast method
Ethidium bromide is a DNA staining dye and can be used to visualize DNA embedded in agarose gel. Both precast…
Cell Line: FTC-236
OVERVIEW Organism Human (Homo sapiens), 42 years old Male Source/Tissue of origin Thyroid, Neck lymph node metastasis, Follicular thyroid…
Composition and Ingredients of DNA Loading Dye
The composition and concentration of DNA loading dye can vary a lot. However, all DNA loading dyes contain two essential…
Preparation of 6X DNA Gel loading buffer (Bromophenol blue, Orange G, Xylene Cyanol FF, EDTA and Ficoll 400)
A 6x DNA Gel loading buffer containing Orange G, Bromophenol blue, Xylene cyanol FF, EDTA and Ficoll 400 is prepared by dissolving 25 mg bromophenol blue, 25 mg xylene cyanol FF, 40 mg Orange G, 1.5 g Ficoll 400 and 1.2 ml of 0.5 M EDTA in 10 mM Tris.Cl, pH 7.6 to a final volume of 10 ml. The final solution (6x DNA loading dye) will contain 0.25% (w/v) bromophenol blue, 0.25% (w/v) xylene cyanol FF, 15% (w/v) Ficoll 400, 60 mM EDTA and 10 mM Tris.
Protocol: Visualizing DNA in Agarose Gel using Ethidium Bromide – Post-electrophoresis Method
Ethidium Bromide is a DNA staining dye and can be used to visualize DNA in agarose gel. Both precast (Ethidium…
Preparation of 6X DNA Gel Loading Dye (Orange G, Bromophenol Blue, Xylene Cyanol FF and Ficoll 400)
OVERVIEW A 6x DNA loading dye containing Orange G, Bromophenol blue, Xylene cyanol FF, and Ficoll 400 is prepared by…
Preparation of 6X DNA Gel Loading Dye (Orange G, Bromophenol Blue, Xylene Cyanol FF and Ficoll 400) Alternative Method
OVERVIEW A 6x DNA loading dye containing Orange G, Bromophenol blue, Xylene cyanol FF, and Ficoll 400 is prepared by…
Cell Line : U3067MG
Overview Organism Human (Homo sapiens), 67 years old, Female Source/Tissue Glioblastoma Morphology Cell type Glioma stem cell /…
U3053MG
Overview Organism Human (Homo sapiens), 64 years old, Male Source/Tissue Glioblastoma Morphology Cell type Glioma stem cell /…
Cell Line : U3068MG
Overview Organism Human (Homo sapiens), 69 years old, Male Source/Tissue Glioblastoma Morphology Cell type Glioma stem cell /…
Nestin
Nestin is an intermediate filament protein Encoded by the NES gene (in human) Found in undifferentiated central nervous system cells…
Preparation of LB-Agar Medium and Plates
OVERVIEW LB medium can be solidified by adding agar. Solidified culture medium is very useful for the selection and identification…
Preparation of 6X DNA Loading Dye (Bromophenol blue and Sucrose)
Overview DNA sample is mixed with the DNA loading dye prior to loading into the wells of agarose gel. A…