Category: Lab Notes

Water Density

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The density of water is dependent on temperature. The maximum density of pure water is 1 g/mL (more precisely 0.999973…

List of Tight Junction Proteins Between Endothelial Cells of Brain Vasculature

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The following tight junction proteins are reported to be present between endothelial cells of brain vasculature: Claudins Claudin-1 Claudin-3  Claudin-5…

Positive Charged Microscope Slides

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Positive Charged Microscope Slides have a good affinity for cells and tissue sections. Biological materials stay adhered to these slides…

Applications of Agarose Gel Electrophoresis

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Agarose gel electrophoresis is extensively used for the analysis of nucleic acid. Observations made by running DNA or RNA samples on agarose gel are very helpful to determine quantity and quality of nucleic acids. These powerful features make agarose gel electrophoresis an important essential step in many well known techniques such as northern and southern blotting, RFLP, RAPD, PCR, molecular cloning etc. Since agarose gel allows the elution of biomolecules, it can also be used for the purification of nucleic acids and large protein molecules and complexes. In addition, it can also be used to analyse biological processes such as apoptosis.

Cell Line : 1022

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Overview Organism Human (Homo sapiens) (Galli et al., 2004) Source/Tissue Primary glioblastoma (Galli et al., 2004) Morphology   Cell type…

Setting up Agarose Gel Electrophoresis in Laboratory

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Agarose gel electrophoresis is a comparatively easy technique and requires limited knowledge & expertise, reagents, instruments and space. KNOWLEDGE AND…

Cell Line : U3088MG

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Overview   Organism Human (Homo sapiens), 67 years old, Female Source/Tissue Glioblastoma Morphology   Cell type Glioma stem cell /…

Beta Tubulin III (Tuj-1)

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Neuronal marker, Specifically expressed in neurons A member of beta tubulin protein family Play important role in neurogenesis, and axon…

Cell Line : U3073MG

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Overview   Organism Human (Homo sapiens), 71 years old, Male Source/Tissue Glioblastoma Morphology   Cell type Glioma stem cell /…

Agarose Gel Electrophoresis of DNA

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DNA agarose gel electrophoresis is an analytical technique of analyzing and separating DNA fragments based on their size. Agarose gel…

Cell Line : D-11

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Overview   Organism Fish – rainbow trout (Oncorhynchus tshawytscha) Source/Tissue Liver Morphology Fibroblastoid Cell type   Growth mode Adherent, monolayer…

Cell Line : U3110MG

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Overview   Organism Human (Homo sapiens), 58 years old, Female Source/Tissue Glioblastoma Morphology   Cell type Glioma stem cell /…

Cell Line : U3082MG

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Overview   Organism Human (Homo sapiens), 70 years old, Female Source/Tissue Glioblastoma Morphology   Cell type Glioma stem cell /…