Category: Lab Notes
Protocol – Thawing and Revival of Cryopreserved Cells
This protocol describes the revival process of cryopreserved adherent cells. The revival process involves rapid thawing of cryopreserved cells followed by removal of freezing medium which contains cryoprotectant, in this case, DMSO. The freezing medium can be removed immediately just after thawing the cells by centrifugation. Alternatively, cells are allowed to adhere to the culture dish followed by washing and addition of fresh growth medium.
Preparation of lysozyme stock solution (10 mg/ml)
Lysozyme is an enzyme used for the extraction of proteins and nucleic acid from bacteria. It improves the extraction process…
Suppliers – 0.5M EDTA solution
Note: We have listed some of the suppliers. You must visit the supplier’s homepage to order and to get more…
Preparation of 0.5 M EDTA Stock Solution from Anhydrous EDTA Free Acid
EDTA (EthyleneDiamineTetraAcetic acid), a polyamino carboxylic acid, is extensively used in molecular biology experiments as a chelating agent. It sequesters…
Suppliers – Ammonia /Ammonium hydroxide solution in water
Note: We have listed some suppliers. You must visit the supplier’s homepage to order and to get more details about…
Comparison of TAE and TBE Electrophoresis Buffers
Both TAE (Tris-Acetate EDTA) and TBE (Tris-Borate EDTA) are common electrophoresis buffers for DNA agarose gel electrophoresis. However, they are quite different in nature, have some useful features and also some disadvantages. You can maximize your results by choosing an electrophoresis buffer depending on your application. The following table lists their properties, and advantages and disadvantages, and also provides explanation for their peculiar behaviour.
Agarose Gel
Agarose gel can be used for the separation of nucleic acids (DNA and RNA) and high molecular weight proteins or protein complexes.
How To Choose Appropriate Cell Line For Research?
Description of basic criteria to select a cell line for your research project.
Protocol – Cryopreservation of Adherent Cells Growing in Serum-free Medium
Overview A serum-free freezing medium is used to preserve cell lines which are maintained in the serum-free growth medium. Serum-free…
Subculturing adherent cells using trypsin-EDTA
Subculturing/passaging can be defined as the preparation of fresh culture by transferring cells from an existing culture. Subculturing is done…
Bacterial Contamination in Cell Culture
Bacterial contamination is one of the most common cell culture contamination. Poor aseptic culture conditions, including handling, incubator and laminar…
Protocol – Subculturing Adherent Cells Growing in Serum-containing Growth Medium using Trypsin-EDTA
Overview The Trypsin-EDTA method, also referred to as trypsinization, is the most commonly used method for passaging/subculturing of adherent cells….
Protocol – Passaging/Subculturing Suspension Culture
Suspension culture is passaged by diluting the existing culture. Since cells float in the medium in suspension culture, they are not treated with a trypsin-EDTA solution. To subculture a suspension cell line, a small amount of cell suspension from the existing culture is transferred to a culture dish containing fresh growth medium.
Passaging/subculturing cells
Subculturing (also called passaging) involves transferring a portion of cells from an existing culture to a new culture dish that…
Passaging/Subculturing Methods for Cells
Several methods have been developed for passaging cells. Each method has its own advantages and drawbacks. Always check the cell…