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Regulation of Anchorage-Independent Growth by Cyclin-dependent Kinase Inhibitors (CKIs)
Cyclin-dependent kinase inhibitors (CKIs), such as p21WAF1, p27KIP1, p16INK4a, or p18INK4c, has been found upregulated in most cell types when…
Regulation of Anchorage-Independent Growth by p27
p27, a member of cyclin-dependent kinase inhibitors (CKIs), is up-regulated in the suspension culture of most cell lines including cancer…
Regulation of Anchorage-Independent Growth by Ras
Expression of dominant active Ras mutant, H-RasV12, has been reported to overcome the requirement of anchorage for a cell to…
Anchorage-Independent Growth
Anchorage independent growth refers to the ability of a cell to grow and proliferate in absence of ECM-integrin interaction. Such…
CRISPR Locus
Please read the following references. REFERENCES Karginov & Hannon, 2010. The CRISPR system: small RNA-guided defense in bacteria and archaea….
Suppliers – Ficoll 400
Note: Ficoll 400 can be purchased from the following suppliers. You must visit the supplier’s product homepage to order and…
Ficoll 400
Synonyms Ficoll 400, Polysucrose-400, Ficoll PM 400q CAS Number 26873-85-8 Chemical structure (C12H20O10)n Molecular Weight ≈ 400,000 Appearance White to…
Agarose Gel Casting Set-ups with no requirement of Sealing Edges of Casting Tray with Tape
Preparing the gel casting tray by sealing both edges (open ends) with tape for agarose gel preparation requires effort. Sometimes agarose solution leaks especially when relatively hot melton agarose is poured into the gel casting tray. To make the agarose gel casting process simple and effortless, many suppliers provide casting tray setups that do not need sealing the edges of casting trays. Some of them are listed in this post.
Suppliers – MOPS Free Acid
Note: MOPS Free Acid can be purchased from the following suppliers. You must visit the supplier’s product homepage to order…
MOPS (4-Morpholinepropanesulfonic acid) Free Acid
IUPAC name 3-morpholin-4-yl propane-1-sulfonic acid Synonym 4-Morpholinepropanesulfonic acid3-(N-Morpholino)propanesulfonic acid3-Morpholinopropanesulfonic acidMorpholinopropanesulfonic acid4-Morpholinopropanesulphonic acid4-Morpholinepropane sulfonic acid3-N-Morpholinopropanesulfonic acid Molecular formula C7H15NO4S Molecular weight…
Preparation of 1M MOPS (Free Acid) Solution
Overview MOPS (Free acid, C7H15NO4S, Molecular weight: 209.26) is a zwitterionic buffer, useful for several cell and molecular biology applications…
Preparation of 10x MOPS Electrophoresis Buffer
OVERVIEW MOPS is a zwitterionic physiological buffer, with pH range 6.5 – 7.9. MOPS-containing electrophoresis buffer is suitable for RNA…
Recommended Agarose Gel Percentage for Efficient Separation of DNA Fragments
Agarose Gel is a porous matrix that acts as a sieve through which negatively charged linear DNA fragments migrate under an…
Molecular Weight and Equivalent Weight of Common Acids and Bases
Chemical name Formula Number of dissociable H+/OH— (valence) in solution Molar mass (= weight weight) g/mol Equivalent weight (g/eq) Hydrochloric…
Cellular Functions of Integrins
Integrins are reported to regulate the following cellular functions. Cell survival and death Cell cycle progression and proliferation Cell shape…