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How to Determine Glioma Cells in Perivascular Space?
Immunostaining using anti-AQP4 and anti-CD31 antibodies can be used to determine glioma cells in perivascular space. Perivascular space is a…
Methods to Visualize Blood Vessels in Brain
Anti-CD31 staining can be used to visualize blood vessels in different body parts including the brain. Immunostaining of fixed brain…
Methods to Identify Vessel-Associated Tumor Cells
Watkins et al., 2014 have used immunostaining-based methods to score vessel associated-glioma cells. Please read Watkins et al., 2014 article…
GBM14
Human patient-derived glioma xenolines (Watkins et al., 2014) REFERENCES: Watkins et al., 2014. Disruption of astrocyte-vascular coupling and the blood-brain…
GBM39
Human patient-derived glioma xenolines (Watkins et al., 2014) REFERENCES: Watkins et al., 2014. Disruption of astrocyte-vascular coupling and the blood-brain…
Blood-Brain Barrier (BBB)
The BBB is a highly specialized structure in the Central nervous system (CNS) between the blood circulation and the brain…
Protocol – Growing Large Volume of Liquid Culture of Escherichia coli for Large-Scale Plasmid Isolation
Large-scale isolation of plasmid requires a large volume of E. coli culture. Large scale plasmid isolation procedures are termed, midiprep (25 – 50 ml starting culture volume) and maxiprep (100 – 500 ml starting culture volume). A starter culture is initially prepared by inoculating a colony in a small volume (2 – 10 ml) of culture medium. Large culture volume is prepared by diluting starter culture in a ratio of 1: 100 to 1: 1000 in the growth medium.
GBM22
Human patient-derived glioma xenolines (Watkins et al., 2014) REFERENCES: Watkins et al., 2014. Disruption of astrocyte-vascular coupling and the blood-brain…
Cell Line : D54MG
Human glioma cell line (Watkins et al., 2014) REFERENCES: Watkins et al., 2014. Disruption of astrocyte-vascular coupling and the blood-brain…
D54MG Glioma Cell Line-Derived Brain Tumors in Immunodeficient Mice
Transplantation of 1.25 – 5.0 x 105 cells in the brain of adult 6 – 12 weeks old immunodeficient C.B.17…
Interaction of Glioma Cells with Pre-existing Blood Vessels (Vessel Co-option)
The interaction of glioma cells with blood vessels has a high impact on glioma development, therefore has been considered as…
Glioma Cell Lines that Show Extensive Perivascular Invasion in Rodent Brain
The following cell lines have been shown to invade perivascularly when transplanted into the rodent brain. D54 (Watkins et al.,…