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Suppliers – 0.5M EDTA solution
Note: We have listed some of the suppliers. You must visit the supplier’s homepage to order and to get more…
Preparation of 0.5 M EDTA Stock Solution from Anhydrous EDTA Free Acid
EDTA (EthyleneDiamineTetraAcetic acid), a polyamino carboxylic acid, is extensively used in molecular biology experiments as a chelating agent. It sequesters…
Preparation of 1 M Tris.Cl Buffer
Overview Tris is also known as THAM, Tris base, Trizma, Tris(hydroxymethyl)aminomethane. Tris is one of the most frequently used buffers…
Preparation of DNase-free Bovine Pancreatic RNase A
Overview Bovine pancreatic RNase (RNase A) is an endoribonuclease. It specifically degrades single-stranded RNA at C and U residues at…
Preparation of 50X TAE Electrophoresis Buffer
A 50x TAE buffer can be prepared by mixing and dissolving 242 g Tris base, 100 ml of 0.5 M EDTA and 57.1 ml glacial acetic acid in a deionized water to a final volume of 1000 ml. The pH of the final solution should be between 8.2 – 8.4.
Preparation of 5X TBE Electrophoresis Buffer
TBE is a very common electrophoresis buffer for DNA agarose gel electrophoresis. It is prepared as a 5X stock solution that can be diluted to 1x or 0.5x working solution for DNA agarose gel electrophoresis. The 5X concentrated solution contains 0.445 M Tris borate and 0.01 M EDTA (pH 8.2 – 8.4).
A 5X stock solution is prepared by dissolving 54 g Tris base, 27.5 g Boric acid, and 20 ml of 0.5 M EDTA in water to a final volume of 1000 ml. The solution is sterilized by autoclaving and is stored at room temperature.
Preparation of Stock Solution of Tetracycline (12 mg/ml)
Overview Tetracycline hydrochloride can be used for biological research. Tetracycline hydrochloride is soluble in water (50 mg/ml) with heating. It…
Ribonuclease A (RNase A) from Bovine Pancreas
Overview CAS Number 9001-99-4 EC Number 232-646-6 Enzyme Commission (EC) Number 3.1.27.5 Synonyms Pancreatic Ribonuclease, RNase A, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase,…
Preparation of Stock Solution of Streptomycin (50 mg/ml)
Overview Streptomycin function by inhibiting protein synthesis in bacteria. Binding of streptomycin to the small 16S rRNA of the 30S…
Preparation of Stock Solution of Kanamycin (50 mg/ml)
Overview Kanamycin is a water-soluble, aminoglycoside bactericidal antibiotic. It was originally purified from the bacterium Streptomyces kanamyceticus. It inhibits protein…
Preparation of LB Lennox Broth
LB medium is a rich growth medium, commonly used to grow E. coli. LB, Lennox Broth contains 1% Bacto-tryptone, 0.5% Bacto-yeast extract and 0.5% Sodium chloride. A 1000 ml LB, Lennox Broth is prepared by dissolving 10 g Bacto-tryptone, 5 g Bacto-yeast extract and 5 g sodium chloride in deionized water to a final volume of 1000 ml. After adjusting the pH 7.0 with 5N NaOH medium is autoclaved and stored in a cold room.
Preparation of Luria-Bertani (LB), Miller Broth
OVERVIEW Luria-Bertani (LB) medium is a rich medium, very commonly used to grow E. coli. It contains tryptone, yeast extract,…
Preparation of Lambda Broth
OVERVIEW Lambda Broth is a moderately rich medium. It is useful in the propagation of λ Phage. It contains tryptone…
Solution
A homogeneous mixture of two or more non-reactive substances is called a solution. A solution is prepared by dissolving one…
Stock Solution
A stock solution can be defined as a highly concentrated solution, mostly prepared as a 10x concentrated solution, which can be diluted to prepare a working solution. Stock solutions can also be used as a component to prepare complex solutions containing many ingredients. For example, a stock solution of EDTA (0.5 M EDTA solution) can be used to prepare many solutions which contain EDTA e.g., TAE, TBE, Tris-EDTA, Trypsin-EDTA etc.