Category: Lab Notes

Plasmid Isolation: Genomic DNA vs Plasmid DNA

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One of the major challenges in plasmid isolation methods is the separation of plasmid DNA from genomic DNA. To better…

Plasmid: pSymA

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Plasmid name pSymA Host Sinorhizobium meliloti Plasmid size(kbp) 1354.2 Plasmid geometry Circular Plasmid copy number 2–3

Plasmid: SCP1

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Plasmid name SCP1 Host Streptomyces coelicolor Plasmid size(kbp) 350.0 Plasmid geometry Linear Plasmid copy number 4

Plasmid: F

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Plasmid name F Host Escherichia coli Plasmid size(kbp) 99.2 Plasmid geometry Circular Plasmid copy number 1–2

Plasmid: lp25

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Plasmid name lp25 Host Borrelia burgdorferi Plasmid size(kbp) 24.2 Plasmid geometry Linear Plasmid copy number 1–2

Plasmid: ColE1

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Plasmid name ColE1 Host Escherichia coli Plasmid size(kbp) 6.6 Plasmid geometry Circular Plasmid copy number 10–30

Plasmid: pUB110

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Plasmid name pUB110 Host Bacillus subtilis Plasmid size(kbp) 2.3 Plasmid geometry Circular Plasmid copy number 20–50

Suppliers: Taq DNA Polymerase

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Note: Taq DNA Polymerase can be purchased from the following suppliers. You must visit the supplier’s product page to order and…

Topic: Aluminium

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Element: Aluminum (Al) Aluminium Chloride [AlCl3] Molecular Weight Calculation Aluminium Bromide [AlBr3] Molecular Weight Calculation Aluminium Fluoride [AlF3] Molecular Weight…

Topic: Silver

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Element: Silver (Ag)  Silver Nanoparticle   Gold Nanoparticles Vs Silver Nanoparticles Silver Sulfide (Ag₂S) Silver Chloride (AgCl) Silver Nitrate (AgNO₃)…

Plasmid: Timeline of Key Events

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1903: The chromosome theory of inheritance was developed independently by two researchers: Walter S. Sutton and Theodor Boveri 1910: The…

Plasmid vs Episome

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Properties Plasmid Episome Remarks Molecular nature DNA DNA Both of them are made up of deoxyribonucleic acid Genetic material type…

Borrelia burgdorferi

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Causes Lyme disease in humans Gram-negative bacteria Microaerophile Motile spirocheteGenome consists of a linear chromosome. It also contains at least…

AGS Cell Line Characteristics

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AGS cell line grows adherently as a monolayer.  This cell line does not have a tendency to form true foci….

35-mm Dishes for Cell Culture

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The 35-mm dishes are small rounded dishes. The diameter of these dishes is 35 mm. However, the diameter (excluding the…