Category: Lab Notes
Suppliers: 95% (w/w) Sulfuric Acid Solution
Note: A 95% (w/w) Sulfuric acid solution can be purchased from the following suppliers. You must visit the supplier’s product…
EcoRV Restriction Enzyme
Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific recognition sites Recognize hexameric palindromic sequence Blunt-end cutter (cut both…
Biological Buffers
Buffer Name pH range pKa at 25°C ACES 6.1 – 7.5 6.78 ADA 6.7 – 7.2 6.59 AMP 9.0 –…
Terminal Deoxynucleotidyl Transferase (TdT)
Template independent DNA polymerase Also known as terminal transferase A member of the X family of DNA polymerase enzymes Add…
Molar – Millimolar Conversion
Formula: 1 molar (M) = 1000 millimolar (mM) More0.001 molar (M) = 1 millimolar (mM)0.01 molar (M) = 10 millimolar…
Calculator: Milliliter – Microliter Conversion
Formula: 1 milliliter (mL) = 1000 microliter (µL) More0.001 milliliter (mL) = 1 microliter (µL)0.01 milliliter (mL) = 10 microliter…
How to Study Changes in Buffer pH During Agarose Gel Electrophoresis?
Changes in electrophoresis buffer pH is an important parameter to consider when optimizing already existing reagents or conditions or testing…
Preparation of 0.5M Rubidium Chloride (RbCl) Stock Solution
A 0.5M stock solution of Rubidium chloride (RbCl, Molecular weight = 120.92) can be prepared by dissolving 6.046 g RbCl in deionized water to a final volume of 100 ml.
TA Cloning
TA cloning involves base pairing of adenine (A) and thymine (T) (complementary base pairs) that result in the joining of…
T-vectors for Cloning
T-vectors are suitable for cloning of DNA fragments that have been amplified by non-proofreading DNA polymerases. These vectors are linear…
ADA (Buffer)
IUPAC name 2-[(2-amino-2-oxoethyl)-(carboxymethyl)amino]acetic acid Synonym N-(2-Acetamido)iminodiacetic acid, N-(Carbamoylmethyl)iminodiacetic acid Molecular formula C6H10N2O5 Linear formula H2NCOCH2N(CH2CO2H)2 Molar mass 190.15 g/mol Appearance…