Category: Lab Notes
Suppliers: Agarose (Standard)
Note: Agarose (standard, CAS No. 9012-36-6) can be purchased from any of the following suppliers. You must visit the supplier’s…
Electroendosmosis in Agarose Gel Electrophoresis
In agarose gel electrophoresis, electroendosmosis (EEO) can be defined as ‘the active water transport towards cathode (negative electrode) from anode…
Preparation of 6X DNA Loading Dye (Xylene Cyanol FF and Ficoll 400)
Overview DNA sample is mixed with DNA loading dye prior to loading into the wells of agarose gel. DNA loading…
Protocol: Size Determination of Plasmid DNA Using Agarose Gel Electrophoresis
Plasmids are circular DNA. Their size can not be determined in their native state as the migration of circular DNA in agarose gel is quite different and can not be compared with the linear fragments of ladder DNA. To know the approximate size of plasmid, the first step is to linearize the plasmid using a restriction enzyme that generates strictly a single cut. This results in a linear DNA that can be run on agarose gel and its size can be compared with the ladder sequence.
Protocol: Size Determination of DNA fragment Using Agarose Gel Electrophoresis
OVERVIEW Approximate size of DNA fragments can easily be determined by agarose gel electrophoresis. This analysis is quite useful to…
Regulation of Anchorage-Independent Growth by Cyclin-dependent Kinase Inhibitors (CKIs)
Cyclin-dependent kinase inhibitors (CKIs), such as p21WAF1, p27KIP1, p16INK4a, or p18INK4c, has been found upregulated in most cell types when…
Regulation of Anchorage-Independent Growth by p27
p27, a member of cyclin-dependent kinase inhibitors (CKIs), is up-regulated in the suspension culture of most cell lines including cancer…
Regulation of Anchorage-Independent Growth by Ras
Expression of dominant active Ras mutant, H-RasV12, has been reported to overcome the requirement of anchorage for a cell to…
Anchorage-Independent Growth
Anchorage independent growth refers to the ability of a cell to grow and proliferate in absence of ECM-integrin interaction. Such…
CRISPR Locus
Please read the following references. REFERENCES Karginov & Hannon, 2010. The CRISPR system: small RNA-guided defense in bacteria and archaea….
Suppliers – Ficoll 400
Note: Ficoll 400 can be purchased from the following suppliers. You must visit the supplier’s product homepage to order and…
Ficoll 400
Synonyms Ficoll 400, Polysucrose-400, Ficoll PM 400q CAS Number 26873-85-8 Chemical structure (C12H20O10)n Molecular Weight ≈ 400,000 Appearance White to…
Agarose Gel Casting Set-ups with no requirement of Sealing Edges of Casting Tray with Tape
Preparing the gel casting tray by sealing both edges (open ends) with tape for agarose gel preparation requires effort. Sometimes agarose solution leaks especially when relatively hot melton agarose is poured into the gel casting tray. To make the agarose gel casting process simple and effortless, many suppliers provide casting tray setups that do not need sealing the edges of casting trays. Some of them are listed in this post.
Suppliers – MOPS Free Acid
Note: MOPS Free Acid can be purchased from the following suppliers. You must visit the supplier’s product homepage to order…