Category: Lab Notes
U3020MG
Human glioma stem cell line (Xie et al., 2015, hgcc.se) REFERENCES Xie et al., 2015. The Human Glioblastoma Cell Culture…
U3019MG
Human glioma stem cell line (Xie et al., 2015, hgcc.se) REFERENCES Xie et al., 2015. The Human Glioblastoma Cell Culture…
How to Determine Glioma Cells in Perivascular Space?
Immunostaining using anti-AQP4 and anti-CD31 antibodies can be used to determine glioma cells in perivascular space. Perivascular space is a…
Methods to Visualize Blood Vessels in Brain
Anti-CD31 staining can be used to visualize blood vessels in different body parts including the brain. Immunostaining of fixed brain…
Methods to Identify Vessel-Associated Tumor Cells
Watkins et al., 2014 have used immunostaining-based methods to score vessel associated-glioma cells. Please read Watkins et al., 2014 article…
D54MG Glioma Cell Line-Derived Brain Tumors in Immunodeficient Mice
Transplantation of 1.25 – 5.0 x 105 cells in the brain of adult 6 – 12 weeks old immunodeficient C.B.17…
Interaction of Glioma Cells with Pre-existing Blood Vessels (Vessel Co-option)
The interaction of glioma cells with blood vessels has a high impact on glioma development, therefore has been considered as…
Glioma Cell Lines that Show Extensive Perivascular Invasion in Rodent Brain
The following cell lines have been shown to invade perivascularly when transplanted into the rodent brain. D54 (Watkins et al.,…
Methods for Detecting Antibiotic-resistant Bacteria
Please read the following peer-reviewed articles to know different methods to detection of antibiotic-resistant bacteria: Ash et al., 2002. Antibiotic…
Preparation of Superbroth
Overview Superbroth contains very high-levels of Tryptone and yeast extract that makes it a super rich medium. It can be…
Preparation of TYGPN Medium
OVERVIEW TYGPN is a rich microbial culture medium. It can be prepared by dissolving 20 g Tryptone, 10 g yeast…
Preparation of NZC Broth
OVERVIEW NZC broth is a rich microbial culture medium. It can be prepared by dissolving 10 g NZ Amine A,…