Category: Lab Notes
Isolation of Glioma Stem Cells from WHO Grade I Gliomas by Neurosphere Assay
There are no reports of successful isolation of glioma stem cells from WHO grade I gliomas by neurosphere assay. Several…
Protocol – Preparation of Gelatin/Chrome Alum-coated Slides for Histological Tissue Sections
Gelatin/Chrome Alum coating helps the slide hold the histological sections firmly, thus reducing the risk of losing histological sections while…
Preparation of Chrome Alum-containing Gelatin Solution for Preparation of Coated Slides for Histological Tissue Sections
Overview:Chrome alum/gelatin coating helps slide to hold the histological sections firmly, thus reducing the risk of losing histological sections while…
Mole Fraction
Mole fraction refers to the fraction of a component of a solution when the amounts (component and solution) are expressed…
Preparation of 10X Dulbecco’s Phosphate buffered saline (DPBS)
Overview: Phosphate buffered saline (PBS) is a buffer solution commonly used in biological research. PBS supports cell viability by keeping…
Preparation of 10X Phosphate Buffered Saline (PBS), Sambrook Method
Overview: Phosphate buffered saline (PBS) is a buffer solution commonly used in biological research. PBS supports cell viability by keeping…
Protocol – Plasmid Isolation by Boiling Method (Miniprep)
Overview The boiling lysis method of plasmid isolation is quick and is recommended for the isolation of small plasmids (up…
Preparation of 5M Sodium Chloride (NaCl)
Overview: NaCl is highly soluble in water. Its aqueous solution is clear and colorless. A 5M NaCl solution is easy…
Sodium Deoxycholate
Synonyms Desoxycholic acid sodium salt, 3α,12α-Dihydroxy-5β-cholanic acid sodium salt, 7-Deoxycholic acid sodium salt, Sodium deoxycholate ChemSpider 82577 PubChem CID 23668196…
Vertical Agarose Gel Electrophoresis for Analysis of High Molecular Weight Proteins
SDS-PAGE does not effectively separate proteins larger than 200 kDa. High molecular weight proteins have difficulties moving and entering small…
Neurogenic Niches in Adult Brain
Two main neurogenic niches in the adult brain have been reported:1. Ventricular-subventricular zone (V-SVZ)2. Subgranular zone of the hippocampus (SGZ)In…
Preparation of 1M Calcium chloride (CaCl2) Stock Solution
OVERVIEW Calcium chloride is a salt of calcium and chlorine. Calcium chloride is available commercially in both anhydrous (CaCl2) and…
Ampicillin Trihydrate
ChemSpider ID22034 PubChem CID 23565 UNII HXQ6A1N7R6 InChIKey RXDALBZNGVATNY-CWLIKTDRSA-N CAS Number 7177-48-2 Molecular formula C16H19N3O4S.3H2O Molecular weight 403.451 g/mol Appearance…
Suppliers – Ampicillin Sodium salt
Ampicillin Sodium salt can be purchased from any of the following suppliers. You must visit the supplier’s homepage to order…