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Protocol – Passaging/Subculturing Suspension Culture
Suspension culture is passaged by diluting the existing culture. Since cells float in the medium in suspension culture, they are not treated with a trypsin-EDTA solution. To subculture a suspension cell line, a small amount of cell suspension from the existing culture is transferred to a culture dish containing fresh growth medium.
Passaging/subculturing cells
Subculturing (also called passaging) involves transferring a portion of cells from an existing culture to a new culture dish that…
Passaging/Subculturing Methods for Cells
Several methods have been developed for passaging cells. Each method has its own advantages and drawbacks. Always check the cell…
Cryopreservation of Cells
Cryopreservation is an efficient way to preserve cells at ultra-low temperatures (below -135°C) which stops all physiological processes and biological aging. It is a routinely used technique in all cell culture laboratories.
Cell Culture
Culturing cells from multicellular organisms especially from animals (mammals, amphibians, insects, birds etc) refers to cell culture or animal cell culture.
Preparation of Freezing Medium Containing DMSO and Serum
Overview The freezing medium is used to preserve cell lines at ultra-low temperatures. This method of preserving cell lines is…
Protocol – Cryopreservation of Adherent Cell Culture
Overview Cryopreservation of adherent cell cultures requires detaching the cells from the substratum, typically through trypsinization. The detached cells are…