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Preparation of Terrific Broth (TB)
Overview: The Terrific Broth was formulated by Tartoff and Hobbs (Tartoff & Hobbs, 1987). It is a rich medium, which…
Preparation of LB Luria Broth
LB is a rich growth medium, commonly used to grow E. coli. LB Luria Broth contains 1% Bacto-tryptone, 0.5% Bacto-yeast extract and 0.05% Sodium chloride. A 1000 ml LB Luria Broth is prepared by dissolving 10 g Bacto-tryptone, 5 g Bacto-yeast extract and 0.5 g sodium chloride in deionized water to a final volume of 1000 ml. After adjusting the pH 7.0 with 5N NaOH medium is autoclaved and stored in a cold room.
Preparation of 5x M9 Minimal Salts
M9 Minimal Salts serve as a base salt solution to prepare M9 Minimal Medium. M9 Minimal salts solution is quite…
Preparation of 20% Glucose Stock Solution
Overview: Glucose is a carbohydrate and is utilized by cells as instant energy and carbon source. In addition, it is…
Preparation of H Medium
Overview H Medium is a moderately rich medium. It contains tryptone and sodium chloride (NaCl). Tryptone provides basic nutrients and…
Preparation of 1 M Glucose Stock Solution
Overview: Glucose is a carbohydrate and is utilized by cells as instant energy and carbon source. In addition, it is…
Immunodeficient NSG (NOD Scid Gamma) Mouse Model
Immunodeficient mice are the most utilized model organisms for the study of several human diseases, especially cancers. The NSG mouse…
LB (Lysogeny Broth) Medium
LB (Lysogeny broth or Luria-Bertani) is the most commonly used growth medium for E. coli culture. LB is a rich medium that contains tryptone, yeast extract and sodium chloride. It promotes fast growth of E. coli in culture by fulfilling all nutritional requirements, thus suitable for all regular molecular biology applications including molecular cloning, plasmid amplification, and recombinant protein production. Several versions of the original recipe formulated by Giuseppi Bertani in 1951 are now in common use: Miller broth, Lennox broth, Luria broth. They differ in NaCl concentration.
Preparation of SOB (Super Optimal Broth) Medium
SOB (Super Optimal Broth) medium is a nutrient-rich microbial growth medium, designed by Douglas Hanahan in 1983. SOB or its glucose-supplemented version SOC is often used during transformation of E. coli competent cells with plasmids (added in transformed cells after heat shock). It results in better survival and higher transformation efficiency as compared to regular LB medium. It contains 2% Bacto-tryptone, 0.5% Bacto-yeast extract, 0.05% Sodium chloride, 2.5 mM KCl, and 10 mM MgCl2. A 1000 ml SOB medium is prepared by dissolving 20 g bacto-tryptone, 5 g bacto-yeast extract, 0.5 g sodium chloride and 2.5 ml of 1M KCl in water to a final volume of 1000 ml. Medium is sterilized by autoclaving. MgCl2 (5 ml of 2M MgCl2) is added just before use.
Preparation of Alkaline Phosphatase Buffer
Overview: An alkaline phosphatase buffer is required for the detection of alkaline phosphatase activity. It contains 5 – 50 mM…
Preparing Working Solution from Stock Solution by Dilution
If you have a concentrated stock solution of known concentration, you can prepare a working solution of specific concentration and…
Glioma Stem Cell (GSC)
Glioma stem cells are a specific type of glioma cell population in glioma tumors that have neural stem cell-like features….
Identifying Human Xenograft in Animal Models Using Immunostaining
There are several antibodies available that specifically recognize human cells and have no cross-reactivity with well-known animal models like mice…
Preparation of 10X Tris-EDTA (TE) Solution
Overview Tris-EDTA (pH 8.0) solution is used for dissolving and storing DNA. Storing DNA in a slightly alkaline condition reduces…
Preparation of Coomassie Brilliant Blue R-250 Solution for Detection of Protein in Acrylamide Gel
Overview: Coomassie Brilliant Blue is a protein stain, which is frequently used to visualize protein on acrylamide gels. Chemically it…