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Phosphate Buffered Saline (PBS)
Phosphate buffered saline (PBS) is a buffer solution commonly used in biological research. PBS is isotonic and non-toxic to cells….
Cancer Stem Cell (CSC)
A specific type of cancer cell population in cancers that has adult stem cell-like features. Features: Ability to Self-renewal Expression…
Isolation of Glioma Stem Cells from WHO Grade I Gliomas by Neurosphere Assay
There are no reports of successful isolation of glioma stem cells from WHO grade I gliomas by neurosphere assay. Several…
Contrast-enhancing vs Non-enhancing Regions (MRI-Imaging) of Glioblastoma
Gill et al., 2014 studied the Glioblastoma cell types in biopsies taken from Contrast-enhancing and non-enhancing regions (MRI-imaging) of human…
Protocol – Preparation of Gelatin/Chrome Alum-coated Slides for Histological Tissue Sections
Gelatin/Chrome Alum coating helps the slide hold the histological sections firmly, thus reducing the risk of losing histological sections while…
Preparation of Chrome Alum-containing Gelatin Solution for Preparation of Coated Slides for Histological Tissue Sections
Overview:Chrome alum/gelatin coating helps slide to hold the histological sections firmly, thus reducing the risk of losing histological sections while…
Mole Fraction
Mole fraction refers to the fraction of a component of a solution when the amounts (component and solution) are expressed…
Preparation of 10X Dulbecco’s Phosphate buffered saline (DPBS)
Overview: Phosphate buffered saline (PBS) is a buffer solution commonly used in biological research. PBS supports cell viability by keeping…
Preparation of 10X Phosphate Buffered Saline (PBS), Sambrook Method
Overview: Phosphate buffered saline (PBS) is a buffer solution commonly used in biological research. PBS supports cell viability by keeping…
Protocol – Plasmid Isolation by Boiling Method (Miniprep)
Overview The boiling lysis method of plasmid isolation is quick and is recommended for the isolation of small plasmids (up…
Preparation of 5M Sodium Chloride (NaCl)
Overview: NaCl is highly soluble in water. Its aqueous solution is clear and colorless. A 5M NaCl solution is easy…
Sodium Deoxycholate
Synonyms Desoxycholic acid sodium salt, 3α,12α-Dihydroxy-5β-cholanic acid sodium salt, 7-Deoxycholic acid sodium salt, Sodium deoxycholate ChemSpider 82577 PubChem CID 23668196…
Vertical Agarose Gel Electrophoresis for Analysis of High Molecular Weight Proteins
SDS-PAGE does not effectively separate proteins larger than 200 kDa. High molecular weight proteins have difficulties moving and entering small…
Migration of Bromophenol Blue and Xylene Cyanol in Agarose Gel Running in TBE or TAE Electrophoresis Buffer
Bromophenol blue and Xylene cyanol are the two most commonly used tracking dyes for the analysis of DNA on agarose gel electrophoresis. These negatively charged dyes not only help in monitoring the progress of agarose gel electrophoresis but also allow easy monitoring of sample loading process onto the wells of agarose gel. Their position in relation to DNA fragments is an important information that helps to determine how far a gel must be run without accidentally letting the DNA fragments of interest run out of the gel and at the same time ensuring good resolution among different size DNA fragments.