Category: Lab Notes
DIPSO
IUPAC name 3-[bis(2-hydroxyethyl)amino]-2-hydroxypropane-1-sulfonic acid Synonym 3-(Bis(2-hydroxyethyl)amino)-2-hydroxy-1-propanesulfonic acid, 3-(N,N-Bis[2-hydroxyethyl]amino)-2-hydroxypropanesulfonic acid, 3-[bis(2-hydroxyethyl)amino]-2-hydroxy-1-propanesulfonic acid, 3-[N,N-Bis(2-hydroxyethyl)amino]-2-hydroxypropanesulfonic Acid Molecular formula C7H17NO6S Molar mass 243.27798 g/mol…
PIPES
IUPAC name 1,4-Piperazinediethanesulfonic acid Synonym Piperazine-N,N?-bis(2-ethanesulfonic acid) Molecular formula C8H18N2O6S2 Molar mass 302.37 g/mol CAS number 5625-37-6 Appearance White powderDecomposes…
TABS
IUPAC Name 4-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]butane-1-sulfonic acid Synonym N-tris(Hydroxymethyl)methyl-4-aminobutanesulfonic acid Empirical formula (Hill system) C8H19NO6S Molar mass 257.30456 g/mol CAS number 54960-65-5 Compound…
Streptococcus thermophilus strain DGCC7710
Streptococcus thermophilus strain DGCC7710 is a model organism to study CRISPR-Cas system (Dupuis et al., 2013). Advantages: Highly active and…
TAPSO
IUPAC name 3-[[1, 3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]-2-hydroxypropane-1- sulfonic acid Synonym 2-Hydroxy-3-[tris(hydroxymethyl)methylamino]-1-propanesulfonic acid, N-[Tris(hydroxymethyl)methyl]-3-amino-2-hydroxypropanesulfonic acid Empirical formula (Hill system) C8H17NO4S Molar mass 259.27738 g/mol…
Lactococcus lactis
Homofermentative bacteria Produces lactic acid from saccharides Used in cheese production Well studied organism
Calculator: Celsius – Fahrenheit conversion
FORMULA F = (C × 9/5) + 32 C = (F-32) x 5/9 C = Celsius F = Fahrenheit CALCULATOR…
TBE (Tris-Borate-EDTA) Electrophoresis Buffer
TBE is one of the very common electrophoresis buffers, used for agarose gel analysis of DNA. It contains Tris, Boric…
Plasmid pSRQ700
Naturally present in Lactococcus lactis subsp. cremoris DCH-4 (Moineau et al., 1995) Size: 7.8 kb (Moineau et al., 1995) Encodes…
HL-60/S4
OVERVIEW Organism Human (Homo sapiens), 36 years, White, Female Source/Tissue of origin Peripheral blood, Acute promyelocytic leukemia Morphology Lymphoblast-like…
Preparation of 10X TBE (Tris-Borate-EDTA) electrophoresis buffer
OVERVIEW Tris-Borate-EDTA electrophoresis buffer is a common electrophoresis buffer for nondenaturing agarose gel electrophoresis. A 10x TBE buffer can be…
Preparation of 10X TB (Tris-Borate) electrophoresis buffer
OVERVIEW EDTA in electrophoresis buffer, although protects DNA from metal-dependent nuclease by sequestering divalent metal ions, also causes heat generation…
POPSO
IUPAC name 2-hydroxy-3-[4-(2-hydroxy-3-sulfopropyl)piperazin-1-yl]propane-1- sulfonic acid Synonym Piperazine-N,N’-bis(2-hydroxypropanesulfonic acid); 1,4-piperazinedipropanesulfonic acid, |A,|A’-dihydroxy-beta,beta’-Dihydroxypiperazine-1,4-dipropanesulphonic acid Empirical formula (Hill system) C10H22N2O8S2 Molar mass 362.42028…
TAPS
IUPAC Name 3-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]propane-1-sulfonic acid Synonym N-[Tris(hydroxymethyl)methyl]-3-aminopropanesulfonic acid, [(2-Hydroxy-1,1-bis(hydroxymethyl)ethyl)amino]-1-propanesulfonic acid Empirical formula (Hill system) C7H17NO6S Molar mass 243.27798 g/mol CAS number…
TEA (Buffer)
IUPAC name 3-[4-(2-hydroxyethyl)piperazin-1-yl]propane-1-sulfonic acid Synonym 4-(2-Hydroxyethyl)-1-piperazinepropanesulfonic acid, 4-(2-Hydroxyethyl)piperazine-1-propanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N?-(3-propanesulfonic acid), HEPPS Empirical formula (Hill system) C9H20N2O4S Molar mass 252.3311…