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Agarose Gel
Agarose gel can be used for the separation of nucleic acids (DNA and RNA) and high molecular weight proteins or protein complexes.
Agarose
Agarose is a useful matrix for a number of analytical and preparative techniques including gel electrophoresis, chromatography, and support matrix to immobilize enzymes and cells. Agarose is available in a variety of forms, which differ in physical properties. The two most common agarose are standard agarose and low melting agarose. Standard agarose is most commonly used for analysis of DNA and RNA. Low melting agarose is often used for preparative purposes such as elution of DNA fragments and complexes.
Preparation of Resuspension Buffer Containing Tris and EDTA for Isolation of Plasmid by Alkaline Lysis Method
Overview: The resuspension buffer is used to resuspend bacterial cells during plasmid isolation by the alkaline lysis method. It provides…
Resuspension Buffer (Solution I) for Isolation of Plasmid by Alkaline Lysis Method
Resuspension buffer (solution I) is used for the isolation of plasmid DNA by alkaline lysis method. Bacterial cells, obtained from…
Basic Steps in Plasmid Isolation
Plasmid isolation is a common task in molecular biology laboratories. Tasks as simple as amplifying a plasmid vector to complex…
Cell Line : A-72
Overview Organism Canine, Dog (Canis familiaris), 8 years, Female, Strain Golden retriever Source/Tissue Tumor, Unknown tissue Morphology Fibroblast-like Cell…
Cell Line : A-10
CELL LINE ORIGIN Organism Rat (Rattus norvegicus) Strain DB1X Age 14–17 day old embryo Source/Tissue Medial and intimal layers of…
Cell Line : A-2
Overview Organism Sweetwater fish – Poeciliid (Xiphophorus xiphidium), Strain: YF1 Source/Tissue Embryonal, Rio Purificacion Morphology Epithelial-like Cell type Embryonal…
Cell Line : A 172
CELL LINE ORIGIN Organism Human (Homo sapiens), 53 years old, Male Source/Tissue Brain, Glioblastoma Year of origin ? CELL LINE…
Abelmoschus esculentus
Abelmoschus esculentus, commonly known as okra or lady’s finger, is a flowering plant belonging to the mallow family (Malvaceae). This…
Molarity of 56.6% Ammonium Hydroxide (28% Aqueous Solution of Ammonia) [NH4OH (NH3 + H2O)]
Ammonium hydroxide (NH4OH) is a clear colorless liquid. A 56.6% (w/w) concentrated ammonium hydroxide can be obtained from different suppliers….
Molarity of 70% (w/w) Nitric Acid (HNO3)
Nitric acid is a clear colorless liquid. A 70% (w/w) concentrated Nitric acid can be obtained from different suppliers. 70%…
Molarity of 95% (w/w) formic acid (HCOOH)
♦ Formic acid is supplied as a clear colorless liquid. A 95% (w/w) concentrated formic acid can be obtained from…