Category: Lab Notes
Diseases caused by Helicobacter pylori
Helicobacter pylori infection has been shown to be highly associated with the following diseases: Gastritis Peptic ulcer Duodenal ulcer Gastric…
Kanamycin
Kanamycin is a broad-spectrum aminoglycoside antibiotic effective against a wide range of gram-negative bacteria, including Escherichia coli, Klebsiella pneumoniae, Proteus…
Escherichia coli K-12
The majority of laboratory strains of E. coli are derived from E. coli K-12 Favorite model organism to study bacterial…
Escherichia coli DH5α
One of the most common laboratory Escherichia coli strains used for molecular cloning Derived from Escherichia coli K-12 High transformation…
Calculator – Calculate Molarity of Concentrated Sodium Hydroxide (NaOH) (w/w)
Calculator – Calculate molarity of concentrated Sodium Hydroxide (NaOH) Use Calculator to calculate the molarity of concentrated Sodium Hydroxide (NaOH)…
Topic: Calcium Chloride (CaCl2)
Preparation of 1M Calcium chloride (CaCl2) Stock Solution Calcium Chloride (CaCl2) Molecular Weight Calculation Calcium Chloride Dihydrate (CaCl2.2H2O) Molecular Weight…
Pichia pastoris
A methylotrophic yeast Discovered in 1960, with the unique ability to utilize methanol as a carbon source and energy A…
Methylotrophs
A diverse group of microorganisms that can utilize single carbon (C1) compounds such as methane, methanol, and methylamine, as a…
Methanol Metabolism by Methylotrophs
The first step of methanol metabolism is the oxidation of methanol which leads to the formation of formaldehyde Formaldehyde is…
Yeast in Biotechnology
Has a long history in biotechnology Safe for humans as it has long been used for Beer production Tools are…
Saccharomyces cerevisiae
Classification Kingdom Fungi Division Ascomycota Subdivision Saccharomycotina Class Saccharomycetes Orders Saccharomycetales Family Saccharomycetaceae Genus Saccharomyces Species cerevisiae Common names: baker’s…
How to Calculate the Molecular Weight of a Compound?
To calculate the molecular weight of any compound, the first step is to know the constituent elements (atoms) and their…
Chloramphenicol Amplification of Low-copy Number Plasmids
Low-copy number plasmid vectors such as those based on pMB1 or ColE1 can be amplified by adding chloramphenicol to the…
Plasmid: pBR322 (Cloning Vector)
Plasmid name pBR322 Application Cloning vector Replicon pMBI Host E. coli DH5α, and other related strains Plasmid size (kbp) 4.361…