Category: Lab Notes
Preparation of 1M Potassium Chloride (KCl) Stock Solution
Overview: Potassium Chloride (KCl) dissolves readily in water. A 1 M stock solution can be prepared by dissolving 74.55 g…
Preparation of 1M Magnesium Sulfate (MgSO4) Stock Solution
Overview Magnesium sulfate (MgSO4) is soluble in water. Magnesium sulfate is available in anhydrous as well as hydrated forms. Since…
Preparation of Terrific Broth (TB)
Overview: The Terrific Broth was formulated by Tartoff and Hobbs (Tartoff & Hobbs, 1987). It is a rich medium, which…
Preparation of 5x M9 Minimal Salts
M9 Minimal Salts serve as a base salt solution to prepare M9 Minimal Medium. M9 Minimal salts solution is quite…
Preparation of 20% Glucose Stock Solution
Overview: Glucose is a carbohydrate and is utilized by cells as instant energy and carbon source. In addition, it is…
Preparation of H Medium
Overview H Medium is a moderately rich medium. It contains tryptone and sodium chloride (NaCl). Tryptone provides basic nutrients and…
Preparation of 1 M Glucose Stock Solution
Overview: Glucose is a carbohydrate and is utilized by cells as instant energy and carbon source. In addition, it is…
Immunodeficient NSG (NOD Scid Gamma) Mouse Model
Immunodeficient mice are the most utilized model organisms for the study of several human diseases, especially cancers. The NSG mouse…
Preparing Working Solution from Stock Solution by Dilution
If you have a concentrated stock solution of known concentration, you can prepare a working solution of specific concentration and…
Glioma Stem Cell (GSC)
Glioma stem cells are a specific type of glioma cell population in glioma tumors that have neural stem cell-like features….
Identifying Human Xenograft in Animal Models Using Immunostaining
There are several antibodies available that specifically recognize human cells and have no cross-reactivity with well-known animal models like mice…
Preparation of 10X Tris-EDTA (TE) Solution
Overview Tris-EDTA (pH 8.0) solution is used for dissolving and storing DNA. Storing DNA in a slightly alkaline condition reduces…
Preparation of Coomassie Brilliant Blue R-250 Solution for Detection of Protein in Acrylamide Gel
Overview: Coomassie Brilliant Blue is a protein stain, which is frequently used to visualize protein on acrylamide gels. Chemically it…
Phosphate Buffered Saline (PBS)
Phosphate buffered saline (PBS) is a buffer solution commonly used in biological research. PBS is isotonic and non-toxic to cells….