Category: Lab Notes
Topic: Cytotoxin-Associated Gene A (CagA)
CagA (Cytotoxin-Associated Gene A) CagA Types Based on EPIYA Motifs EPIYA Motifs CagA – PAR1b/MARK2 Interaction CagA/E-cadherin interaction Phosphorylation-Independent CagA…
Nucleolus
The nucleolus is the most prominent membrane-less subnuclear structure found in all eukaryotic cells during interphase, although it disappears during…
M Phase (Cell Cycle)
Proliferating mammalian cells undergo drastic morphological changes. A well-spread adherent cell suddenly loses its contact from the surroundings, rounds up,…
Passage Number
Passage number refers to the number of times cells have been subcultured or passaged i.e., upon confluence, cells are transferred…
Importance of Subculturing in Cell Culture
Cell culture is not static. Cells acquire changes when maintained in culture for an extended period (Masters & Stacey, 2007)….
Papain Digestion of Antibody/Immunoglobulin
Partial digestion of antibodies (immunoglobulins) with the protease papain produces three fragments. Among these, two identical fragments are known as…
Antibody Structure
Antibodies belong to the immunoglobulin superfamily. A typical immunoglobulin, such as IgG, is a large protein made up of four…
Cell Line: NCI-H1882
Cell Line: NCI-H1882 Synonyms: NCI-H1882 NCIH1882 H1882 H-1882 Culture Type: Cell line Keywords: Human Small Cell Lung Cancer Cell Line…
Cell Line: NCI-H2009
Cell Line: NCI-H2009 Synonyms: NCI-H2009 NCIH2009 H2009 H-2009 Culture Type: Cell line Keywords: Human Lung Adenocarcinoma Cell Line Description: Established…
Curing Mycoplasma Infected Culture
Mycoplasma contaminated culture can either be discarded or be cured. The best approach is to discard the culture because: It…
Contaminating Mycoplasma species in Cell Culture
Several mycoplasma species from human, porcine and bovine origin have been detected in cell culture. The following eight species account…
Preventing Mycoplasma Contamination in Cell Culture
Mycoplasma contamination is a common and challenging problem in cell culture laboratories, but the following measures can substantially reduce the…
Sources of Mycoplasma Contamination
Mycoplasma contamination is a prevalent issue in cell culture laboratories, posing significant challenges. However, a comprehensive understanding of mycoplasma contamination…
Common Mycoplasma Species Detected in Cell Culture
The following species of mycoplasma are most frequently detected in cell culture: Mycoplasma pirum Mycoplasma salivarium Mycoplasma orale Mycoplasma fermentans…
Effects of Mycoplasma on Cultured Cells
Mycoplasma can affect cell culture in a variety of ways. These are: Morphological changes Slow proliferation rate Reduced saturation density…