Author: admin
Klenow Fragment
A 68kDa C-terminal fragment of E. coli DNA Polymerase I The Klenow fragment is a large proteolytic fragment of DNA…
T4 DNA Polymerase
Overview Template-dependent DNA polymerase Catalyzes 5′-3′ synthesis from primed single-stranded DNA Possesses 5’→3′ polymerase activity Possesses 3′-5′ exonuclease activity Lacks…
Tfl DNA Polymerase
Isolated from the thermophilic eubacterium Thermus flavus Thermostable DNA polymerase Exhibits a 5′→3′ exonuclease activity Lacks a 3′→5′ exonuclease activity…
Taq DNA Polymerase
Overview: Isolated from the thermophilic bacterium Thermus aquaticus Thermostable DNA polymerase Catalyzes 5’→3′ DNA synthesis (5’→3′ polymerase activity) from primed…
Thermostable DNA Polymerases
OVERVIEW Can withstand high temperature Active at high temperature Important tools for polymerase chain reaction Examples of Thermostable DNA Polymerases…
Preparation of 1M Hydrofluoric Acid from Concentrated Stock Solution (48%, w/w)
OVERVIEW A 48% (w/w) Hydrofluoric acid can be purchased from several commercial suppliers. It is a clear colorless liquid and…
Molarity of 48% (w/w) Hydrofluoric Acid (HF)
OVERVIEW A 48% (w/w) Hydrofluoric acid (HF) is a clear colorless aqueous solution of Hydrogen fluoride (HF) gas. It can…
Suppliers: 48% (w/w) Hydrofluoric Acid (HF)
Note: 48% (w/w) Hydrofluoric acid (HF) can be purchased from the following suppliers. You must visit the supplier’s product page…
BfuAI Restriction Enzyme
Endonuclease Type IIS Restriction enzyme Cleaves double-stranded DNA outside of specific recognition site Recognizes asymmetric DNA sequences (see fig) DNA…
Preparation of 1M Perchloric Acid (HClO4) From Concentrated Stock Solution (70%, w/w)
OVERVIEW A 70% (w/w) perchloric acid can be purchased from several commercial suppliers. It is a clear colorless liquid and…
Suppliers: 70% (w/w) Perchloric Acid (HClO4)
Note: 70% (w/w) Perchloric acid (HClO4) can be purchased from the following suppliers. You must visit the supplier’s product page…
Molarity of 70% (w/w) Perchloric Acid (HClO4)
OVERVIEW A 70% (w/w) Perchloric acid is a clear colorless aqueous solution of HClO4. It can be purchased from many…
Suppliers: 95% (w/w) Formic Acid (HCOOH)
Note: 95% (w/w) Formic acid can be purchased from the following suppliers. You must visit the supplier’s product page to…
Preparation of 1M Formic acid from Concentrated Stock Solution (95%, w/w)
OVERVIEW A 95% (w/w) Formic acid can be purchased from several commercial suppliers. It is a clear colorless liquid and…
Preparation of 1M Potassium hydroxide from Concentrated Stock Solution (45%, w/w)
OVERVIEW A 45% (w/w) Potassium hydroxide can be purchased from several commercial suppliers. It is a clear colorless liquid and…