Category: Lab Notes

Mycoplasma contamination is still a challenging problem in most cell culture laboratories. Due to their small size, they escape microscopic…

Cell Line: NCI-H2110 Synonyms: NCI-H2110 NCIH2110 H2110 H-2110 Culture Type: Cell line Keywords: Human Non-small Cell Lung Cancer Cell line …

Cell Line: NCI-H295R Synonyms: NCI-H295R NCIH295R H295R H-295R Culture Type: Cell line Keywords: Human Adrenocortical Carcinoma Cell Line Description: Parent…

Cell Line: NCI-H211 Synonyms: NCI-H211 NCIH211 H211 H-211 Culture Type: Cell line Keywords: Human Small Cell Lung Carcinoma Cell Line…

HCC827 – A Human epithelial-like lung adenocarcinoma (NSCLC) cell line, established from the lung of a white, 39-year-old female patient with adenocarcinoma.

Synonyms: 293-PERV-PK-CIRCE Description: Parent cell line: 293 (also known as HEK-293) Infected with endogenous retrovirus of pigs, produced by the…

Description: HEK293-A cells (subclone of HEK-293) stably expressing GFP-tagged human WIPI1 Synonyms: HEK293-A GFP-WIPI1 Keywords: HEK293-A cells (a subclone of…

Notes: Cryopreservation  Applications of Cryopreservation Cryopreservation of Cells  Natural Cryopreservation   Detrimental Effects of Freezing on Cells and Tissues Temperature Effects…

To cryopreserve, cells from the suspension culture are harvested by centrifugation and resuspended at the correct cell density in an appropriate freezing medium. The resuspension is aliquoted into cryovials and subjected to slow freezing overnight followed by transfer to either the vapor phase of liquid nitrogen or a -150°C freezer. 

A clear colorless liquid in the ventricles and subarachnoid spaces of the brain Produced by the choroid plexus in the…

Cryopreservation (Cryo: icy cold or frost; Preservation: Storage without deterioration) refers to the storage of living material at ultra-low temperatures below -135°C without losing its vitality in a manner that stops biological time and aging.

The nuclear membrane acts as a barrier between the cytoplasm and the nucleoplasm. Under certain situations, the nuclear membrane is…

Cell proliferation refers to a process of cell growth and cell division into two daughter cells. Cell proliferation leads to an exponential increase in cell number and tissue size.

MCF7 cells are reported to form tumors in Nude mice in an estrogen-dependent manner (Osborne et al., 1985) Osborne et…

The following proteins can be detected in human plasma. Acid phosphatase 3 ADAM metallopeptidase with thrombospondin type 1 motif 13…